Summary
We used A chain extracted from urine of patient with myeloma (IgD λ) as antigen for immunizing BALB/C mice, and 86 hybridoma cell clones secreting monoclonal antibodies (McAb) were obtained after fusing twice and cloning 3–4 times. 15 of these clones secreted monoclonal anti-idiotypic antibodies (anti-Id McAb). The results showed that 12 of 15 anti-Id antibodies reacted only with homologous λ chain and IgD, not with A chain, κ chain, IgG, IgA, IgM, IgD, IgE, albumin and paraglobulin from normal subjects. Indirect immunofluorescent assay demonstrated that positive reaction rate between anti-Id McAbs and peripheral blood lymphocytes or bone marrow cells of the patient with myeloma was up to 23 %. No reaction was observed between anti-Id McAb and peripheral blood lymphocytes or bone marrow cells from normal subjects. Some of these McAbs presented positive reaction with plasmacytoma cell lines cultured in our laboratory.
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References
1990;19(5):309–11.
1988;4(5): 299–302.
Sun B, et al. Studies on monoclonal anti-idiotypic antibodies to human B cell leukemia. J Tongji Med Univ 1989; 9(4):244–50.
Pillemer E, Weissmann IL. A monoclo-nal antibody that detects a Vk-Tepc15 idiotypic determinant. Cross-reactive with a thy-1 determinant. J Exp Med 1981;153:1068–79.
Dörken B, et al. Therapie mit mono-klonalen antidiotypischen Antikörpern bei einem Patient mit progredientem Immunzytom. Klin Wochenschr 1986; 64(SV):105.
Mayumi M, et al. Studies on the clonal origin of human B cell leukemia using monoclonal anti-idiotype antibodies. J Immunol 1982;129:904–10.
Wrightham M, et al. Monoclonal anti-light chain idiotype as a tumor specific probe for human neoplastic B lymphocytes. Blood 1987;63(3):919–23.
Tutt AL, et al. Antibodies against urinary light chain idiotypes as agents for detection and destruction of human neoplastic B lymphocytes. J Immunol 1983;131(6):3058–60.
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Xiao-lin, W., Hui-fen, Z., Yue, Z. et al. Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: I. study on monoclonal anti-idiotypic antibodies against λ chain protein of myeloma. Journal of Tongji Medical University 10, 169–175 (1990). https://doi.org/10.1007/BF02986457
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DOI: https://doi.org/10.1007/BF02986457