Abstract
The anti-allergic actions of the leaves ofCastanea crenata, (Fagaceae) were studied. The water extract demonstrated potent anti-allergic actions inin vivo andin vitro experiments. The oral or intraperitoneal administration of the extract (100 or 200 mg/kg) caused a significant inhibition of the 48 hr-PCA (up to 90%) and the vascular permeability induced by histamine or serotonin in rats (about 80%). The anaphylactic release of β-hexosaminidase from RBL-2H3 cells was also significantly inhibited by the extract in a dose-dependent manner with an IC50 value of 230 μg/ml. The activity-guided fractionation of the extract, based on the, determination of inhibitory effect upon the release of β-hexosaminidase, led to the isolation of quercetin as an active principle responsible for the inhibition of degranulation.
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Lee, E., Choi, E.J., Cheong, H. et al. Anti-allergic actions of the leaves ofCastanea crenata and isolation of an active component responsible for the inhibition of mast cell degranulation. Arch Pharm Res 22, 320–323 (1999). https://doi.org/10.1007/BF02976372
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DOI: https://doi.org/10.1007/BF02976372