Abstract
The effect of ischemia/reperfusion-induced neuronal damage on the memory impairment were investigated using active avoidance and Morris water maze tasks in Wistar rats. Focal ischemia was induced by 1 h occlusion of the right middle cerebral artery (MCA) of Wistar male rats. Reperfusion was induced by releasing the occlusion and restoring the blood circulation for 24 h. The acquisition and preservation memory tested by active avoidance showed a significant difference between the sham and ischemia/reperfusion group. The water maze acquisition performance was also significant difference between sham and ischemia/reperfusion groups in both latency and moving distance. The infarction volume was increased by the ischemia/reperfusion. Furthermore, the cresyl violet staining of the ischemia/reperfusion brain showed severe neuronal damage (pyramidal cell loss) in the cortex in addition to the striatum lesion of brain. This study shows that pyramidal cell damage in the cortex lesion may be partially related to memorial disturbance in the ischemia/reperfusion brain injury.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dunnett, S. B., Everitt, B. J., Robbins, T. W., The basal forebrain-cortical cholinergic system: interpreting the functional consequences of excitotoxic lesions.Trends Neurosci., 14(11), 494–501 (1991).
Hirakawa, M., Tamura, A., Nagashima, H., Nakayama, H., Sano, K., Disturbance of retention of memory after focal cerebral ischemia in rats.Stroke, 25(12), 2471–2475 (1994).
Hong, J. T., Ryu, S. R., Kim, H. J., Kim, D. B., Lee, J. K., Him, Y. J., Lee, S. H., Lee, B. M., Kim, P. Y., Protective effect of green tea on the ischemia-reperfusion-induced oxidative brain injury in wistar rats.Toxicol. Lett., 109, 82 (1999).
Kataoka, K., Hayakawa, T., Kuroda, R., Yuguchi, T., Yamada, K., Cholinergic deafferentation after focal cerebral infarct in rats.Stroke, 22(10), 1291–1296 (1991).
Petito, C. K., Morgello, S., Felix, J. C., Lesser, M. L., The two patterns of reactive astrocytosis in postischemic rat brain.J. Cereb. Blood Flow Metab. 10(6), 850–859 (1990).
Pulsinelli, W., Excitotoxic damage in global ischemia.Adv. Neurol., 71, 62–67 (1996).
Rolls, E. T., Memory systems in the brain.Annu. Rev. Psychol., 51, 599–630 (2000).
Scremin, O. U., Jenden, D. J., Effects of middle cerebral artery occlusion on cerebral cortex choline and acetylcholine in rats.Stroke, 20(11), 1524–1530 (1989).
Spangler, E. L., Heller, B., Hengemihle, J., Muthm, N. J., Jones, B. E., Garofalo, P., Ingram, D. K., Thrombosis of parietal, but not striate, cortex impairs acquisition of a 14-unit T-maze in the rat.Physiol. Behav., 56(1), 95–101 (1994).
Squire, L. R.,Memory and Brain, Oxoford University Press, Oxoford, Egland, pp56–74, 1987.
Takagi, N., Miyake, K., Taguchi, T., Tamada, H., Takagi, K., Sugita, N., Takeo, S., Failure in learning task and loss of cortical cholingergic fibers in microsphere-embolized rats.Exp. Brain Res., 114(2), 279–287 (1997).
Tamura, A., Graham, D. I., McCulloch, J., Teasdale, G. M., Focal cerebral ischaemia in the rat: 1. Description of technique and early neuropathological consequences following middle cerebral artery occlusion.J. Cereb. Blood Flow Metab., 1(1), 53–60 (1981).
Yamamoto, M., Tamura, A., Kirino, T., Shimizu, M., Sano, K., Behavioral changes after focal cerebral ischemia by left middle cerebral artery occlusion in rats.Brain Res., 14, 323–328 (1988).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hong, J.T., Ryu, S.R., Kim, H.J. et al. Involvement of cortical damage in the ischemia/reperfusion-induced memory impairment of wistar rats. Arch Pharm Res 23, 413–417 (2000). https://doi.org/10.1007/BF02975457
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02975457