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Immunomodulatory effects ofBacillus firmus on mouse peritoneal cellsin Vitro

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Abstract

The effect of nonpathogenic G+ bacteriumB. firmus (BF) on stimulation of mouse peritoneal cellsin vitro was evaluated by testing nitric-oxide-synthesis induction and cytokine formation. The reactivity was compared of peritoneal cells from two inbred mouse strains, C57B1/6 and BALB/c, which differ in their immunological reactivity. Peritoneal macrophages from C57B1/6 produced more nitric oxide after a 1-d cultivation with inactivated BF than those of BALB/c mice. In both strains, production can be further increased by adding exogenous IFN-γ to the culture. There were no significant differences between peritoneal cells of these two mouse strains in cytokine production after optimalin vitro stimulation with BF. BF effectively activated peritoneal cells for the production of TNF-α, IL-1β and IL-10, delipidated bacterium (DBF) being more efficient than BF in induction of IL-10 and TNF-α. On the other hand, BF had only small effect on IFN-γ production and no detectable effect on IL-12 production. Macrophage activation by BF/DBF can represent one of the mechanisms responsible for previously described immunomodulatory activity of BF.

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Abbreviations

BF:

Bacillus firmus

DBF:

delipidatedBacillus firmus

FCS:

fetal calf serum

IFN-γ:

interferon γ

IL:

interleukin

LPS:

lipopolysaccharide

NDCM:

Nocardia-delipidated cell mitogen

PBS:

phosphate-buffered saline

TNF:

tumor necrosis factor

References

  • Barot-Ciorbaru R., Brochier J., Miyawaki T., Preud’homme J.L., Petit J.F., Bona C., Taniguchi N., Revillard J.P.: Stimulation of human B lymphocytes byNocardia-derived cell mitogen and derived fractions fromN. opaca. Structure-activity relationship.J.Immunol. 35, 3277–3283 (1985).

    Google Scholar 

  • Gately M.K., Carvajel D.M., Connaughton S., Gilessen R.R., Warrier K.D., Kolinsky K.D., Wilkinson V.L., Dwyer C.M., Higgins G.F.J., Podlaski D.A.: Interleukin-12 antagonist activity of mouse interleukin-12 p40 homodimerin vitro andin vivo.Ann.N.Y.Acad.Sci. 795, 1–12 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Havlickova M., Prokesova L., Zanvit P., Tacner J., Limberkova R.: Adjuvant effect ofBacillus firmus in intranasal immunization of guinea pigs with inactivated type B infuenza virus.Folia.Microbiol. 51, 154–156 (2006).

    Article  CAS  Google Scholar 

  • Hieny S., Scharton-Kertson T., Jankovic H., Charost H., Germain R.N., Sher A.:In vivo microbial stimulation induces rapid Cd40 ligand-independent production of interleukin-12 by dendritic cells and their redistribution to T cell areas.J.Exp.Med. 186, 1819–1829 (1997).

    Article  PubMed  Google Scholar 

  • Klimp A.H., de Vries E.G., Scherphof G.L., Daemen T.: A potential role of macrophage in the treatment of cancer.Crit.Rev.Oncol.Hematol. 44, 143–161 (2002).

    Article  PubMed  CAS  Google Scholar 

  • Kmoníčková E., Zídek Z.: Quantitative aspects of lipopolysaccharide and cytokine requirements to generate nitric oxide in macrophages from LPS-hyporesponsive (Lps d) C3H/HeJ mice.Folia Microbiol. 49, 737–744 (2004).

    Article  Google Scholar 

  • Le Pape A., Barot-Ciorbaru R., Musset M., Baulieu J.L., Jubault C., Lemarie E., Pourcelot L., Besnard J.C., Nicolau C., Mathe G.: An original method for submacroscopic metastases visualization in case of cancer residual minimal disease.Biomed.Pharmacother. 40, 392–398 (1986).

    PubMed  Google Scholar 

  • Mára M., Julák J., Menčíková E., Očenášková J., Dohnalová A.: Effect of crude lipids on the course ofListeria infection in mice.Folia Microbiol. 37, 455–460 (1992).

    Article  Google Scholar 

  • Mára M., Otčenášková J., Nováková M., Julák J., Menčíková E.: Resistance to infection and activation of the monocyte-macrophage system cause byBacillus firmus and its fractions.Folia Microbiol. 39, 147–151 (1994).

    Article  Google Scholar 

  • Marletta M.A., Yoon P.S., Iyengar R., Leaf C.D., Wishnok J.S.: Macrophage oxidation ofl-arginine to nitrate: nitric oxide is an intermediate.Biochemistry 27, 8706–8711 (1988).

    Article  PubMed  CAS  Google Scholar 

  • Mills C.D., Kincaid K., Alt J.M., Heilman M.J., Hill A.M.: M-1/M-2 macrophages and TH1/TH2 paradigm.J.Immunol. 164 6166–6173 (2000).

    PubMed  CAS  Google Scholar 

  • Mlčková P., Čechová D., Chalupná P., Novotná O., Prokešová L.: Enhanced systemic and mucosal antibody response to a model protein antigen after intranasal and intratracheal immunisation usingBacillus firmus as an adjutant.Immunol.Lett. 77, 39–45 (2001).

    Article  PubMed  Google Scholar 

  • Mlčková P., Čechová D., Marušková L., Chalupná P., Novotná O., Prokešová L.: Role of T cells in the adjutant effect ofBacillus firmus in the immune system of mice: intranasal and intratracheal immunization study with ovalbumin.Folia Microbiol. 48, 427–434 (2003).

    Article  Google Scholar 

  • Mlčková P., Poláček M., Čechová D., Marušková L., Staňkova I., Chalupná P., Novotná O., Julák J., Prokešová L.: Intratracheal and intranasal immunization with ovalbumin conjugated withBacillus firmus as a carrier in mice.Folia Microbiol. 50, 247–254 (2005).

    Article  Google Scholar 

  • Prokešová L., Nováková M., Mára M.: Effect ofBacillus firmus and other sporulating aerobic microorganisms onin vitro stimulation of human lymphocytes. A comparative study.Folia Microbiol. 39, 501–504 (1994).

    Article  Google Scholar 

  • Prokešová L., Mlčková P., Staňková I., Chloubová A., Novotná V., Ladmanová P., Chalupná P., Mára M.: Effect ofBacillus firmus on antibody formation after mucosal and parenteral immunization in mice.Immunol.Lett. 64, 161–166 (1998).

    Article  PubMed  Google Scholar 

  • Prokešová L., Mlčková P., Staňková I., Ladmanová P., Ježková J., Chalupná P., Novotná O., Čechová D., Julák J.: Immunostimulatory effect ofBacillus firmus on mouse lymphocytes.Folia Microbiol. 47, 193–197 (2002).

    Article  Google Scholar 

  • Skeen M.J., Miller M.A., Shinnick T.M., Ziegler H.K.: Regulation of murine macrophages IL-12 production.J.Immunol. 156, 1196–1206 (1996).

    PubMed  CAS  Google Scholar 

  • Štěpánková R., Mára M., Očenášková J.: Prolonged survival of ANV Wistar rats with transplated Yoshida sarcoma and increase of granular lymphocytes after administration ofBacillus firmus and their crude lipids.Folia Microbiol. 40, 413–416 (1995).

    Article  Google Scholar 

  • Sutterwala F.S., Noel G.J., Clynes R., Mosser D.M.: Selective suppression of interleukin-12 induction after macrophage receptor ligation.J.Exp.Med. 185, 1977–1985 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Trebichavský I., Barot-Ciorbaru R., Charley B., Šplíchal I.: Induction of inflammatory cytokines byNocardia fractions.Folia Biol. 39, 243–249 (1993).

    Google Scholar 

  • Zanvit P., Havlíčková M., Táčner J., Jirkovská M., Petrásková P., Novotná O., Čechová D., Julák J., Šterzl I., Prokešová L.: Immune response after adjuvant mucosal immunization of mice with inactivated influenza virus.Immunol.Lett. 97, 251–259 (2005).

    Article  PubMed  CAS  Google Scholar 

  • Zídek Z., Tučková L., Mára M., Barot-Ciorbaru R., Prokešová L., Tlaskalová H.: Stimulation of macrophages byBacillus firmus: production of nitric oxide and cytokines.Internal.J.Immunopharmacol. 20, 359–368 (1998).

    Article  Google Scholar 

  • Zídek Z., Franková D., Boubelík M.: Genetic variation inin vitro cytokine induced production of nitric oxide by murine peritoneal macrophages.Pharmacogenetics 10, 493–501 (2000).

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, 128 00, Prague, Czechia

    I. Lomáková, P. Petrásková, I. Šterzl & L. Prokešová

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  1. I. Lomáková
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  2. P. Petrásková
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  3. I. Šterzl
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  4. L. Prokešová
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Corresponding author

Correspondence to L. Prokešová.

Additional information

This work was supported by theMinistry of Education, Youth and Sports of the Czech Republic (grant MSM 002 162 0806) and by theGrant Agency of the Czech Republic (grant 310/03/H147).

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Lomáková, I., Petrásková, P., Šterzl, I. et al. Immunomodulatory effects ofBacillus firmus on mouse peritoneal cellsin Vitro . Folia Microbiol 51, 243–247 (2006). https://doi.org/10.1007/BF02932130

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