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Stimulation of pigment accumulation inAnabaena azollae strains: Effect of light intensity and sugars

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Abstract

The influence of high light intensity on the growth and pigment accumulating ability ofAnabaena azollae was investigated.A. azollae responded positively to high light intensity (6 klx) and was further evaluated at higher intensities (10 and 15 klx), in the presence of glucose, sucrose and jaggery ± DCMU. Significant enhancement in phycobiliproteins and carotenoids was observed in the sugar supplemented cultures at high light intensities. SDS-PAGE profiles of whole cell proteins revealed the presence of unique bands in such treatments. Sucrose supplementation induced a 30–90% increase in carotenoids, phycocyanin and phycoerythrin content at 10 klx. Molecular analysis of the stimulatory and interactive role of sugars on pigment enhancement at high light intensity may aid in better exploitation of cyanobacteria as a source of pigments.

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Abbreviations

ARA:

acetylene-reducing activity

APC:

allophycocyanin

Chl:

chlorophyll

D:

dark

DCMU:

3-(3′,4′-dichlorophenyl)-1,1-dimethylurea

DG:

dark + glucose

DGD:

dark + glucose + DCMU

DM:

dry mass

DMRT:

Duncan’s multiple range test

L:

light

LG:

light + glucose

LGD:

light + glucose + DCMU

LH:

high light intensity

LJ:

light + jaggery

LJD:

light + jaggery + DCMU

LL:

low light intensity

LS:

light + sucrose

LSD:

light + sucrose + DCMU

PBS:

phycobiliproteins

PC:

phycocyanin

PE:

phycoerythrin

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Authors and Affiliations

  1. Center for Conservation and Utilization of Blue-Green Algae, Indian Agricultural Research Institute, 110 012, New Delhi, India

    V. Venugopal, R. Prasanna, A. Sood & P. Jaiswal

  2. Division of Microbiology, Indian Agricultural Research Institute, 110 012, New Delhi, India

    B. D. Kaushik

Authors
  1. V. Venugopal
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  2. R. Prasanna
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  3. A. Sood
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  4. P. Jaiswal
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  5. B. D. Kaushik
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Corresponding author

Correspondence to R. Prasanna.

Additional information

This work was supported by grants fromIndian Council of Agricultural Research andCouncil of Scientific and Industrial Research (New Delhi, India).

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Venugopal, V., Prasanna, R., Sood, A. et al. Stimulation of pigment accumulation inAnabaena azollae strains: Effect of light intensity and sugars. Folia Microbiol 51, 50–56 (2006). https://doi.org/10.1007/BF02931450

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