Abstract
Considerable variation in counts of antibiotic-resistant enterobacteria in the ovine gastrointestinal tract was observed. The occurrence of ruminal and fecal isolates resistant to ampicillin (Ap), kanamycin (Km) and tetracycline (Tc) culminated in summer months, followed by rapid decline in subsequent months. Using PCR thetem1 bla (Apr),aphA1 (Kmr) andtetB (Tcr) genes were found to be predominant. Underin vitro conditions all resistance genes were transferable into laboratoryEscherichia coli strain with relatively high frequency (10−3 transconjugants per recipient).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aminov R.I., Chee-Sanford J.C., Garrigues N., Teferedegne B., Krapac I.J., White B.A., Mackie R.I.: Development, validation, and application of PCR primers for detection of tetracycline efflux genes of Gram-negative bacteria.Appl.Environ.Microbiol.4, 1786–1793 (2002).
Benczeová S., Adam D., Vrábelová M., Michálková-Papajová D., Kettner M.: Occurrence of endemic plasmids causing β-lactam resistance inEnterobacteriaceae in Children’s University Hospital in Munich.Folia Microbiol.49, 457–464 (2004).
Birnboim H.C., Doly J.: A rapid alkaline extraction procedure for screening recombinant plasmid DNA.Nucl.Acids Res.7, 1513–1522 (1979).
Courvalin P., Davies J.: Antimicrobials: time to act!Curr.Opin.Microbiol.6, 425–426 (2003).
Drahovská H., Slobodníková L., Kocíncová D., Seman M., Končeková R.: Antibiotic resistance and virulence factors among clinical and food enterococci isolated in Slovakia.Folia Microbiol.49, 763–768 (2004).
Ewans P.D., Cook S.N., Riggs P.D., Noren C.J.: LITMUS (TM): multipurpose cloning vectors with a novel system for bidirectionalin vitro transcription.Biotechniques19, 130–135 (1995).
Ewing W.H.: Edwards and Ewing’s identification ofEnterobacteriacae, 4th ed., Elsevier, New York 1986.
Frana T.S., Carlson S.A., Griffith R.W.: Relative distribution and conservation of genes encoding aminoglycoside-modifying enzymes inSalmonella enterica serotype Typhimurium phage type DT104.Appl.Environ.Microbiol.1, 445–448 (2001).
Franiczek R., Krzyżanowska B., Dolna I., Mokracka G., Szufnarowski K.: Extended-spectrum β-lactamase-conferring transferable resistance to different antimicrobial agents inEnterobacteriaceae isolated from bloodstream infections.Folia Microbiol.50, 119–124 (2005).
Hungate R.E.: The rumen microbial ecosystem.Ann.Rev.Ecol.Syst.6, 39–46 (1975).
Kudva I.T., Hatfield P.G., Hovde C.J.:Escherichia coli O157:H7 in microbial flora of sheep.J.Clin.Microbiol.34, 431–433 (1996).
Levesque C., Piche L., Larose C., Roy P.H.: PCR mapping of integrons reveals several novel combinations of resistance genes.Antimicrob.Agents Chemother.39, 185–191 (1995).
Malík R., Pristaš P., Javorský P.: Occurrence of plasmid mediated ampicillin resistance among enterobacteria from the ovine rumen.Folia Microbiol.49, 187–190 (2004).
Morrison M.: Do ruminal bacteria exchange genetic material?J.Dairy Sci.79, 1476–1486 (1996).
Pansegrau W., Miele L., Lurz R., Lanka E.: Nucleotide sequence of the kanamycin resistance determinant of plasmid RP4: homology to other aminoglycoside 3′-phosphotransferases.Plasmid18, 193–204 (1987).
Sansonetti P.: Molecular and cellular mechanisms of invasion of the intestinal barrier by enteric pathogens. The paradigm ofShigella.Folia Microbiol.43, 239–246 (1998).
Scott K.P., Flint H.J.: Transfer of plasmids between strains ofEscherichia coli under rumen conditions.J.Appl.Bacteriol.78, 189–193 (1995).
Shaw K.J., Rather P.N., Hare R.S., Miller G.H.: Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.Microbiol.Mol.Biol.Rev.3, 138–163 (1993).
Šustáčková A., Nápravníková E., Schlegelová J.: Antimicrobial resistance ofEnterococcus spp. isolates from raw beef and meat Products.Folia Microbiol.49, 411–417 (2004).
Stefani S., Agodi A.: Molecular epidemiology of antibiotic resistance.Internat.J.Antimicrob.Agents13, 143–153 (2000).
Van Den Bogaard A.E., Stobberingh E.E.: Epidemiology of resistance to antibiotics links between animals and humans.Internat.J.Antimicrob.Agents14, 327–335 (2000).
Author information
Authors and Affiliations
Additional information
This work was supported byVEGA Grant Agency (grant no. 2/5140/25) and theSlovak Agency for Support of Science and Technology (grant APVT-51-012602). We thank Dr. S. Kocherginskaya (University of Illinois, Urbana, USA) for kind gift of bacterial strain harboring the pRT11 plasmid.
Rights and permissions
About this article
Cite this article
Malík, R., Ivan, J., Javorský, P. et al. Seasonal dynamics of antibiotic-resistantEnterobacteriaceae in the gastrointestinal tract of domestic sheep. Folia Microbiol 50, 349–352 (2005). https://doi.org/10.1007/BF02931417
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02931417