Abstract
In submergedClaviceps cultures the activity of hydroxymethylglutaryl-CoA reductase preceded the increase of alkaloid production and of sterol content. During the first alkaloid phase, cell mevalonate was involved in the biosynthesis of both alkaloids and steroids. In the second production phase, it was predominantly used for alkaloid synthesis. Hydroxymethylglutaryl-CoA reductase appears to be a suitable target for physiological manipulation to increase clavine alkaloid yields.
Similar content being viewed by others
Abbreviations
- HMG-CoA:
-
3-hydroxy-3-methylglutarylcoenzyme A
References
Birch A.J., McLouglin B.J., Smith H.: The biosynthesis of the ergot alkaloids.Tetrahedron Lett. 7, 1–3 (1960).
Cardemil E., Jabalaquinto A.M.: The mechanism of action of mevalonate-5-pyrophosphatedecarboxylase.Trends Biochem.Sci. 8, 7–8 (1983).
Dickerson A.G., Mantle P.G., Szczyrbak C.A.: Autolysis of extracellular glucans producedin vitro by a strain ofClaviceps fusiformis.J.Gen.Microbiol. 60, 403–415 (1970).
Feingold K.R., Wiley M.H., Macrae G., Kaysen G., Schoenfeld P.Y., Siperstein M.D.: The effect of uremia on circulating mevalonate metabolism in rats.Metabolism 32, 215–296 (1983).
Floss H.G.: Biosynthesis of ergot alkaloids: Stereochemistry of hydrogen elimination from C-4 of mevalonate.Chem.Commun. 804–805 (1967).
Hall R.N.: N6-(Δ2-Isopentenyl)adenosine: chemical reactions, biosynthesis and fuction of tRNA.Progr.Nucl.Acid Res.Mol.Biol. 10, 57–86 (1970).
Hart M.C., Emerson H.: β-Ergostenol.J.Amer.Chem.Soc. 54, 1070–1074 (1932).
Henry S.A.: Membrane lipids of yeast: Biochemical and genetic studies, pp. 101–158 inThe Molecular Biology of the Yeast Saccharomyces (J.N. Strathern, E.W. Jones, J.R. Broach, Eds). Cold Spring Harbor Lab., New York 1982.
Křen V., Pažoutová S., Rylko V., Sajdl P., Wurst M., Řeháček Z.: Extracellular metabolism of sucrose in a submerged culture ofClaviceps purpurea: Formation of monosaccliarides and clavine alkaloids.Appl.Environ.Microbiol. 48, 826–829 (1984).
Křen V., Řezanka T., Sajdl P., Řehaček Z.: Identification of sterols in submerged cultures of the differentClaviceps species.Biochem. Physiol. Pflanzen. 181, 505–510 (1980).
Lee S.L., Floss H.G., Heinstein P.: Purification and properties of dimethylallyl pyrophosphate: tryptophan dimethylallyltransferase, the first enzyme of ergot alkaloid biosynthesis inClaviceps sp. SD 58.Arch.Biochem.Biophys. 177, 84–94 (1976).
Longley R.P., Rose A.H., Knights B.A.: Composition of the protoplast membrane fromSaccharomyces cerevisiae.Biochem.J. 108, 401–412 (1968).
Pažoutová S., Flieger M., Sajdl P., ŘeháČek Z., Taisinger J., Bass A.: The relationship between intensity of oxidative metabolism and predominance of agroclavine or elymoelavine in submergedClaviceps purpurea cultures.J.Nat.Prod. 44, 225–235 (1981).
Pažoutová S., Slokoska L.S., Nikolova N., Angelov T.I.: Sugar and phosphate metabolism and alkaloid production phases in submerged cultures of twoClaviceps strain.Evr.J.Appl. Microbiol.Biotechnol. 16, 208–211 (1982).
Quain D.E., Haslam J.H.: The effects of catabolite depression on the accumulation of steryl esters and the activity of β-hydroxymethylglutaryl-CoA reductase inSaccharomyces cerevisiae.J.Gen.Microbiol. 111, 343–351 (1979).
Quesney-Huneeus V., Galick H.A., Siperstein M.D.: The dual role of mevalonate in the coll cycle.J.Biol.Chem. 258, 378–385 (1983).
Řeháček Z.: New trends in ergot alkaloid synthesis.Process Biochem. 18, 22–30 (1983)
Robebs J.E., Robertson L.W., Hornemann K.M., Jindra A., Floss H.G.: Physiological studies on the induction of alkaloid synthesis by tryptophan and its inhibition by phosphate.J.Bacteriol. 112, 791–796 (1972).
Robertson W.L., Robbers J.E., Floss H.G.: Some characteristic of tryptophan uptake inGlaviceps species.J.Bacterial. 114, 208–219 (1973).
Schmauder H.P., GrögeeD.: Untersuchungen zur Aromatenbiosynthese und AlkaloidbildungClaviceps in Arten.Biochem.Physiol.Pflanzen 164, 41–47 (1973).
Schmauder H.P., Gröger D.: Chitinbestimmung inClaviceps.Biockem. Physiol. Pflanzen 173, 139–145 (1978).
Wurst M., Flieger M., ŘeháČek Z.: Analysis of ergot alkaloids by high-performance liquid chromatography. I. Clavines and simple derivatives of lysergic acid.J.Chromatogr. 150, 477–483 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Křen, V., Pažoutová, S., Rylko, V. et al. Saprophytic production of Clavine alkaloids and activity of Hydroxymethylglutaryl-CoA reductase. Folia Microbiol 31, 282–287 (1986). https://doi.org/10.1007/BF02926952
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02926952