Abstract
Neither exit nor counterflow efflux of thiamin, taken up previously by an active transport, were found inSaccharornyces cerevisiae, in either the wild type or a mutant with a lower rate of thiamin phosphorylation. Complete inhibition of thiamin phosphorylation by oxythiamin did not lead to any release of thiamin taken up by the cell.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Armstrong W.McD.: Surface active agents and cellular metabolism. The effect of cationic detergents on the production of acid and carbon dioxide by baker’s yeast.Arch. Biochem. Biophys. 71, 137–147 (1957).
Hotchkiss R.D.: The nature of bacterial action of surface active agents.Ann. N. Y. Acad. Sci. 46, 479–493 (1946).
Iwashima A., Nose Y.: Inhibition of thiamine transport in anaerobic baker’s yeast by iodoaoetate, 2, 4-dinitrophenol, N, N’-dicyclohexylcarbodiimide and fatty acids.Biochim. Biophys. Acta 399, 375–383 (1975).
Iwashima A., Nishhto H., Nose Y.: Carrier-mediated transport of thiamine in baker’s yeast.Biochim. Biophys. Acta 330, 222–234 (1973).
Iwashima A., Nishimura H., Nose Y.: Soluble and membrane bound thiamine-binding proteins fromSaccharomyces cerevisiae.Biochim. Biophys. Acta 557, 460–468 (1979).
Iwashima A., Nishimura H., Sempuku K.: Active transport of dimethialium inSaccharomyces cerevisiae.Experientia 36, 385–386 (1980).
Kawasaki T., Miyata I., Esaki K., Nose Y.: Thiamine uptake inEscherichia coli. I. General properties of thiamine uptake system inEscherichia coli.Arch. Biochem. Biophys. 131, 223–230 (1969).
Kawasaki T., Yamada K.: The uptake system of free thiamine in mutants ofEscherichia coli.Biochem. Biophys. Res. Commun. 47, 465–471 (1972).
Manney T.R.: Action of a super-suppressor in yeast in relation to allelic mapping and complementation.Genetics 50, 109–121 (1964).
Nakayama H., Hayashi R.: Biosynthesis of thiamine pyrophosphate inEscherichia coli.J. Bacteriol. 109, 936–939 (1972).
Nishimune T.: Thiamine transport and accumulation inStaphylococcus aureus cells. III. Free thiamine accumulation without phosphorylation.Vitamins 47, 221–227 (1973).
Nishimune T., Tomita M., Yoshii Z., Hayashi R.: Counterflow efflux of thiamine inEscherichia coli.Biochim. Biophys. Acta 649, 419–426 (1981).
Nishimune T., Tomita M., Hayashi R.: On the mechanism of accumulation of free thiamine inEscherichia coli.Vitamins 56, 19–25 (1982).
Ruml T.: Study of thiamine excretion in mutants ofSaccharomyces cerevisiae. PhD Thesis, Institute of Chemical Technology, Prague, 1984.
Rydon H.N.: Note on an improved method for the preparation of oxythiamine.Biochem. J. 48, 383–384 (1951).
Šilhánková L.: Method for preparation of vitaminB1 US Pat. 4 186 252 (1980).
Šilhánková L.: Genetic control of thiamine excretion and of its supression inSaccharomyces cerevisiae.J. Inst. Brew. 91, 238–241 (1985).
Yamada K., Kawasaki T.: Properties of the thiamine transport system inEscherichia coli.J. Bacteriol. 141, 254–261 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ruml, T., Šilhánková, L. & Rauch, P. The irreversibility of thiamin transport inSaccharornyces cerevisiae . Folia Microbiol 33, 372–376 (1988). https://doi.org/10.1007/BF02925847
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02925847