Abstract
Multigram quantities (2.5–10 g) of highly purified IgG were obtained within 4 h from serum by using Avid AL™ packed in a radialflow column. Avid AL™ is an affinity gel containing a synthetic, lowmol-wt ligand capable of selectively binding IgG from serum of all animal species tested. By packing the gel in a radial-flow column up to 500 mL, a high flow rate of 50 mL/min can be achieved without adversely affecting the performance of the gel and the purity of the isolated antibody.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Duhamel, R. C., Schur, P. H., Brendel, K., and Meezan, E. (1979),J. Immunol Methods 31, 211.
Goding, J. W. (1969),Monoclonal Antibodies: Principles and Practice, p. 119.
Moellering, B. J., and Prior, C. P. (1990),Biopharm. 3, 34.
Fahnestock, S. R. (1987),Trends Biotechnol. 5, 79.
Boyle, M. D. P. (1984),BioTechniques Nov./Dec, 334.
Mcguire, J. (1989),Am. Biotech. Lab. I, 28.
Porath, J., Maisano, F., and Belew, M., (1985),FEBS Letter 185, 306.
Hutchens, T. W., and Porath, J. (1986),Anal. Biochem. 159, 217.
Porath, J. (1987),Biopolymers 26, S193.
Porath, J., and Belew, M. (1987),Trends Biotechnol. 5, 225.
Belew, M., Junti, N., Larsson, A., and Porath, J. (1987),J. Immunol. Methods 102, 173.
Nopper, B., Kohen, F., and Wilchek, M. (1989),Anal. Biochem. 180, 66.
Ngo, T. T., and Khatter, N. (1990),J. Chromatog. 510, 281.
Khatter, N., Matson, R. S., and Ngo, T. T. (1991),Am. Lab. in press.
Ngo, T. T. (1991), US Patent 4,981,961.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ngo, T.T., Khatter, N. Rapid and simple isolation of multigram goat IgG from serum using avid aL™ and radial flow column. Appl Biochem Biotechnol 30, 111–119 (1991). https://doi.org/10.1007/BF02922027
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02922027