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Regulation of phosphotransferases in glucose- and xylose-fermenting yeasts

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Abstract

This research examined the titers of hexokinase (HK), phosphofructokinase (PFK), and xylulokinase (XUK) inSaccharomyces cerevisiae and two xylose fermenting yeasts,Pachysolen tannophilus andCandida shehatae, following shifts in carbon source and aeration. Xylose-grown C.shehatae, glucose-grownP. tannophilus, and glucose-grownS. cerevisiae, had the highest specific activities of XUK, HK, and PFK, respectively. XUK was induced by xylose to moderate levels in both P.tannophilus and C.shehatae, but was present only in trace levels inS. cerevisiae. HK activities in P.tannophilus were two to three fold higher when cells were grown on glucose than when grown on xylose, but HK levels were less inducible in C.shehatae. The PFK activities inS. cerevisiae were 1.5 to 2 times higher than in the two xylose-fermenting yeasts. Transfer from glucose to xylose rapidly inactivated HK in P.tannophilus, and transfer from xylose to glucose inactivated XUK in C.shehatae. The patterns of induction and inactivation indicate that the basic regulatory mechanisms differ in the two xylose fermenting yeasts.

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Authors and Affiliations

  1. Institute for Microbial and Biochemical Technology, Forest Products Laboratory, One Gifford Pinchot Drive, 53705-2398, Madison, WI

    Vina W. Yang & Thomas W. Jeffries

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  1. Vina W. Yang
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  2. Thomas W. Jeffries
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Maintailied in cooperation with the University of Wisconsin, Madison, WI

This article was written and prepared by U.S. Government employees on official time, and it is therefore in the public domain and not subject to coypright.

The use of trade, firm or corporation names in this publication is for the information and covenience of the reader. Such use does not constitute an official endorsement or approval by the U.S. Department of Agriculture of any product or service to the exclusion of others which may be suitable.

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Yang, V.W., Jeffries, T.W. Regulation of phosphotransferases in glucose- and xylose-fermenting yeasts. Appl Biochem Biotechnol 63, 97–108 (1997). https://doi.org/10.1007/BF02920416

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