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Cadmium uptake kinetics in human erythrocytes

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Abstract

Cross-membrane transport of cadmium in human erythrocytes was studied using109Cd++ and liquid scintillation counting. Uptake rates were determined by depletion of radioactivity in the incubation medium and the amount of hemolyzate radioactivity taken up by the erythrocytes. Both saturable and nonsaturable components for cadmium transport were observed.

The mean maximum uptake rate (Jmax) of the saturable component was 4.9×10−6 mol/L/h. The transport constant (Kt) was estimated at 6.9×10−5 mol/L. The diffusion constant (Kd) of the nonsaturable component was 1.4×10−2/h. Both Jmax and Kt of cadmium generally decreased when Zn++ or Cu++ was present, with a biphasic response in the presence of Cu++. Kd of cadmium increased as Zn++ or Cu++ levels were increased.

It is suggested that cadmium may penetrate human red cells via cation transport sites owing to its behavior as an analog of one or more nutrient species.

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References

  1. H. A. Schafer, Characteristics of municipal waste water discharges,Annual Report, Southern California Coastal Water Research Project, 1976, pp. 57–60.

  2. Citizens for a Better Environment Report.Toxics in the bay: An assessment of the discharge of toxic pollutants to the San Francisco Bay by municipal and industrial point sources (1983).

  3. J. O. Nriagu,Cadmium in the Environment, Part I, Wiley, New York, NY, 1980, p. 73.

    Google Scholar 

  4. US Environmental Protection Agency,Technical and microanalysis of cadmium and its compounds, EPA, Office of Toxic Substances, Rep. EPA 560/3-75-005, Washington, DC (1975).

    Google Scholar 

  5. H. A. Schroeder, A. P. Nason, I. H. Tipton, and J. J. Balassa,J. Chron. Dis. 20, 179 (1967).

    Article  PubMed  CAS  Google Scholar 

  6. K. Schwarz and J. Spallholz,Fed. Proc. 35, 255 (1976).

    Google Scholar 

  7. M. N. Gleason, R. S. Gosselin, H. C. Hodge, and R. P. Smith,Clinical Toxicology of Commercial Products: Acute Poisoning (Home and Farm), Williams and Wilkins, Baltimore, MD (1969).

    Google Scholar 

  8. W. M. Gafafer,Occupational Diseases, a Guide to Their Recognition, US Department of Health, Eduation, and Welfare, Public Health Services, Washington, DC, 1964, p. 103.

    Google Scholar 

  9. E. C. Hise and W. Fulkerson,Cadmium, the Dissipated element, W. Fulkerson and H. E. Goelher, eds., Oak Ridge National Library, 1973, pp. 203–278.

  10. L. Friberg, M. Piscator, G. F. Nordberg, and T. Kjellstrom,Cadmium in the Environment, 2nd ed., CRC Press, Cleveland OH, 1974, p. 128.

    Google Scholar 

  11. L. Friberg,Acta Med. Scand. 138, 124 (1950).

    Google Scholar 

  12. T. J. Smith, T. L. Petty, J. C. Reading, and A. Lakshminarayan,Am. Rev. Resp. Dis. 114, 161 (1976).

    PubMed  CAS  Google Scholar 

  13. G. Kazantzis, F. V. Flynn, J. S. Spowage, and D. G. Trott,Quart. J. Med. 32, 165 (1963).

    PubMed  CAS  Google Scholar 

  14. R. Lauwerys, J. P. Buchet, H. A. Roels, and D. Stanescu,Arch. Environ. Health 28, 145 (1974).

    PubMed  CAS  Google Scholar 

  15. R. Lauwerys, J. P. Buchet, and H. A. Roels,Int. Arch. Occup. Environ. Health 36, 275 (1976).

    Article  PubMed  CAS  Google Scholar 

  16. R. G. Adams, J. F. Harrison, and P. Scott,Quart. J. Med. 38, 425 (1969).

    PubMed  CAS  Google Scholar 

  17. M. Pujol, J. Arlet, R. Bollinelli, and P. Carles,Arch. Mal. Prof. Med. Trav. Secur. Sco. 31, 637 (1970).

    CAS  Google Scholar 

  18. F. Princi,J. Ind. Hyg. Toxicol. 29, 315 (1947).

    CAS  Google Scholar 

  19. A. Ishizaki, M. Fukushima, and M. Sakamoto,Jap. J. Hyg. 25, 207 (1970).

    PubMed  CAS  Google Scholar 

  20. C. V. Reynolds and E. B. Reynolds,J. Assoc. Public Anal. 9, 112 (1971).

    CAS  Google Scholar 

  21. M. R. S. Fox,Trace Elements in Human Health and Disease, vol. 2: Essential Toxic Elements, A. S. Prasad and D. Oberleas, eds., Academic, New York, NY, 1976, pp. 401–416.

    Google Scholar 

  22. R. E. Duggan and P. E. Corneliussen,Pestic. Monit. J. 5, 331 (1972).

    PubMed  CAS  Google Scholar 

  23. E. E. Menden, V. J. Elia, L. W. Michael, and H. G. Petering,Environ. Sci. Technol. 6, 830 (1972).

    Article  CAS  Google Scholar 

  24. S. J. Kopp, T. Glonek, M. H. Perry, M. Erlanger, and E. F. Perry,Science 217, 837 (1982).

    Article  PubMed  CAS  Google Scholar 

  25. A. Furst and M. C. Schlauder,J. Environ. Pathol. Toxicol. 1, 51 (1977).

    CAS  Google Scholar 

  26. E. Rivedal and T. Sanner,Cancer Res. 41, 2950 (1981).

    PubMed  CAS  Google Scholar 

  27. R. J. Rubin and R. Kroll,Toxicologist 1, 25 (1981).

    Google Scholar 

  28. C. L. Potts,Ann. Occup. Hyg. 8, 55 (1965).

    PubMed  CAS  Google Scholar 

  29. L. N. Kolonel,Cancer Res. 37, 1782 (1976).

    CAS  Google Scholar 

  30. T. J. Oberly, C. E. Piper, and D. S. McDonald,J. Toxicol. Environ. Health 9, 367 (1982).

    Article  PubMed  CAS  Google Scholar 

  31. B. C. Casto, J. Meyers, and J. A. DiPaolo,Cancer Res. 39, 193 (1979).

    PubMed  CAS  Google Scholar 

  32. J. A. DiPaolo and B. C. Casto,Cancer Res. 39, 1008 (1979).

    PubMed  CAS  Google Scholar 

  33. J. D. Heck and M. Costa,Biol. Trace Elem. Res. 4, 71 (1982).

    Article  CAS  Google Scholar 

  34. J. W. Drake,Nature 221, 1132 (1969).

    Article  PubMed  CAS  Google Scholar 

  35. T. A. Kunkel and L. A. Loeb,J. Biol. Chem. 254, 5718 (1979).

    PubMed  CAS  Google Scholar 

  36. S. K. Niyogi, R. P. Feldman, and D. J. Hoffman,Toxicology 22, 9 (1981).

    Article  PubMed  CAS  Google Scholar 

  37. H. Kubinski,Proc. Am. Assoc. Cancer Res. 20, 223 (1979).

    Google Scholar 

  38. A. Furst and S. B. Radding,J. Environ. Sci. Health C2, 103 (1984).

    Google Scholar 

  39. P. M. May, P. W. Linder, and D. R. Williams,J. Chem. Soc., 588 (1977).

  40. O. J. Lucis, M. E. Lynk, and R. Lucis,Arch. Environ. Health 18, 307 (1969).

    PubMed  CAS  Google Scholar 

  41. G. F. Nordberg,Environ. Physiol. Biochem. 2, 7 (1972).

    CAS  Google Scholar 

  42. P. W. Washko and R. J. Cousins,J. Nutrition 107, 920 (1977).

    CAS  Google Scholar 

  43. J. M. Frazier and B. S. Kingsley,Clinical chemistry and chemical toxicology of metals, S. S. Brown, ed., Elsevier, Amsterdam 1977, pp. 33–36.

    Google Scholar 

  44. D. A. Cataldo, T. R. Garland, and R. E. Wildung,Plant Physiol. 73, 844 (1983).

    Article  PubMed  CAS  Google Scholar 

  45. H. G. Ferreira and V. L. Lew,Nature (London)259, 47 (1976).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Harney Science Center, Department of Biology, University of San Francisco, 94117-1080, San Francisco, CA

    Quan H. Nguyen & Paul K. Chien

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  1. Quan H. Nguyen
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  2. Paul K. Chien
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Nguyen, Q.H., Chien, P.K. Cadmium uptake kinetics in human erythrocytes. Biol Trace Elem Res 22, 119–129 (1989). https://doi.org/10.1007/BF02916643

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