Abstract
The presence and restriction fragment length polymorphism (RFLP) of DNA fragments hybridizing with virulence and “house keeping” gene probes were analyzed for 87 group B streptococcal (GBS) strains of human and bovine origin. Most characteristics obtained for bovine strains were similar when compared with those for human strains. The most significant degree of RFLP was discovered for the sizes ofHindIII fragments containingbca gene. Human GBS strains withbac gene, encoding β antigen with IgA binding capacity, were characterized by almost identical complex hybridization patterns with multiple gene probes. At the same timebac gene was not found among bovine GBS strains. GenescpB that encodes C5a peptidase in all human GBS strains was detected only in 9 of 39 strains of bovine origin. These two characteristics effectively distinguished bovine GBS strains from GBS strains of human origin.
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References
Baker C.J., Edwards M.S.: Group B streptococcal infections, pp. 742–811 in I.S. Remington, J.O. Klein (Eds):Infectious Diseases of the Fetus and Newborn Infant., Saunders, Philadelphia (PA) 1990.
Bentley R.W., Leigh J.A.: Determination of 16S ribosomal RNA gene copy number inStreptococcus uberis, S. agalactiae, S. dysgalactiae andS. parauberis.FEMS Immunol. Med. Microbiol.12, 1–7 (1996).
Chmouryguina I.I., Suvorov A.N., Ferrieri P., Cleary P.P.: Conservation of C5a peptidase genes in group A and B streptococci.Infect. Immun.64, 2387–2390 (1996).
Denning D.V., Bacer C.J., Troup N.J., Tompkins L.S.: Restriction endonucleases analysis of human and bovine group B streptococcus for epidemiologic study.J. Clin. Microbiol.27, 1352–1356 (1989).
Dmitriev A., Suvorov A., Totolian A.: Physical and genetic chromosomal maps ofStreptococcus agalactiae, serotypes II and III; rRNA operon organization.FEMS Microbiol. Lett.167, 33–39 (1998).
Forsman P., Tilsala-Timisjarvi A., Alatossava T.: Identification of staphylococcal and streptococcal causes of bovine mastitis using 16S-23S rRNA spacer regions.Microbiol. Read.143, 3491–3500 (1997).
Heden L.O., Frithz E., Lindahl G.: Molecular characterization of an IgA receptor from group B streptococci: sequence of the gene, identification of a proline-rich region with unique structure and isolation of N-terminal fragments with IgA-binding capacity.Eur. J. Immunol.21, 1481–1490 (1991).
Jerstrom P.J., Chhatwal G.S., Timmis K.N.: The IgA bindingb antigen of the C protein complex of group B streptococci: sequence determination of its gene and detection of two binding regions.Mol. Microbiol.5, 843–849 (1991).
Maniatis T., Fritsch E.F., Sambrook J.:Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor (NY) 1982.
Michel J.L., Madoff L.C., Olson K., Kling D.E., Kasper D.L.: Large, identical tandem repeating units in the C protein α antigen gene,bca, of group B streptococci.Proc. Natl. Acad. Sci. USA89, 10060–10064 (1993
Smola J.: Pig and nutria as hosts ofStreptococcus agalactiae, p. 121 inAbstr. 12th LancefieM Internat. Syrup. Streptococci and Streptococcal Diseases, Saint Petersburg (Russia) 1993.
Suvorov A., Dmitriev A., Utinovich I., Schalen C., Totolian A.: Molecular analysis of clinical group B streptococcal strains by use of α and β gene probes.FEMS Immunol. Med. Microbiol.17, 149–154 (1997).
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Dmitriev, A., Tkáčiková, L., Suvorov, A. et al. Comparative genetic study of group B streptococcal strains of human and bovine origin. Folia Microbiol 44, 449–453 (1999). https://doi.org/10.1007/BF02903721
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DOI: https://doi.org/10.1007/BF02903721