Summary
The cell cycle kinetics of uninfected and feline leukemia virusinfected canine lymphoma cell lines were determined by autoradiography (PLM method) as follows:DT-5: generation time (Tc), 15.2 h; pre-synthetic gap phase (TG1), 3.2 h; DNA-synthetic phase (Ts), 8.2 h; post-synthetic gap ph se (TG2), 3.3 h; visible mitotic phase (TM), 0.5 h.11028: Tc, 13.6 h; TG1,1.9 h; Ts, 7.7 h; TG2, 3.4 h; TM, 0.6 h.11028+FeLV (11028 productively infected with feline leukemia virus): Tc, 11.2 h; TG1, 0.2 h; Ts, 8.3 h; TG2, 2.1 h; TM, 0.6 h. Exposure of the lymphoma cell lines to methotrexate (MTX) in vitro produces dose-related increases in cellular volume, associated with reductions in cellular proliferation. The relative sensitivities of these cell lines to MTX, measured by the ID50 MTX concentrations for DT-5, 11028, and 11028 + FeLV are 118nM, 122 nM, and 28 nM respectively. The cell kinetic effects of the ID50 MTX concentrations added to cultures of lymphoma cells pulse-labeled with tritiated thymidine are an approximately 2-h prolongation of Tc, attributable to a lengthening of Ts, with other cell cycle phases not significantly altered. These cell lines are highly tumorigenic when transplanted into the cheek pouches of immunosuppressed hamsters, with inocula of 104 cells repeatably producing rapidly growing, well vascularized tumors.
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This investigation was supported in part by a grant from the Mid-America Cancer Center Program, and submitted in partial fulfillment of requirements for a Ph.D. degree in the Department of Anatomy, University of Kansas Medical Center. The authors wish to acknowledge Dr. David Dennison and Dr. John Clancy, Jr. (Department of Anatomy, University of Kansas Medical Center) for access to and training in the use of the Coulter Model ZBI particle counter
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Torres, J., Klein, R.M., Tung, HN. et al. Cell cycle kinetics of uninfected and feline leukemia virus-infected canine lymphoma cell lines: Effects of methotrexate treatment. Virchows Archiv B Cell Pathol 33, 139–153 (1980). https://doi.org/10.1007/BF02899177
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DOI: https://doi.org/10.1007/BF02899177