Abstract
Subcellular fractions containing plasma membranes with bound DNA and associated proteins were isolated from two different cultures ofBacillus subtilis 168 growing exponentially at different rates. Differences in the contents of individual proteins between the dissociated complexes, established electrophoretically, can be explained by dynamic binding of the proteins to DNA, resulting in a control of DNA replication. Electron microphotographs of isolated complexes display, in addition to unit membranes, associated filamentous structures in different arrangement. Patterns obtained after treating the complexes with nucleases suggest a polydeoxyribonucleotide character of the filamentous structures.
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References
Anagnostopoulos C., Spizizen J.: Requirements for transformation inBacillus subtilis.J. Bacterial.81, 741 (1961).
Chow L. T., Davidson N.: Electron microscope mapping of the distribution of ribosomal genes of theBacillus subtilis chromosome. J. Mol. Biol.75, 265 (1973).
Dingman C. W.: Bidirectional chromosome replication: some topological considerations.J. Theor. Biol.43, 187 (1974).
Dische Z.: Some new characteristic color tests for thymonucleic acid and a microchemical method for determining the same in animal organs by means of these tests.Mikrochemie8, 4 (1930).
Dische Z.:The Nucleic Acids, Vol. I, p. 285. Chargaff E., Davidson J. N. (Eds), Academic Press, New York (1955).
Farmer J. L., Rothman J.: Transformable thymine-requiring mutant ofBacillus subtilis.J. Bacteriol.89, 262 (1965).
Ferrandes B., Frehel C., Chaix P.: Fractionnement et purification des systemes membranaires cytoplasmiques et mesosomiques deBacillus subtilis. Etude de quelques-unes de leurs proprietes oxydoreductrices associees a la chaine respiratoire.Biochem. Biophys. Acta223, 292 (1970).
Fielding P., Fox C. F.: Evidence for stable attachment of DNA to membrane at the replication origin ofEscherichia coli.Biochem. Biophys. Res. Commun.41, 157 (1970).
Fujita H., Komano T., Maruyama Y.: Dynamic aspects of membrane-bound DNA inBacillus subtilis during the course of synchronous growth.Biochem. Biophys. Res. Commun.52, 1361 (1973).
Ganesan A. T., Lederberg J.: A cell-membrane bound fraction of DNA.Biochem. Biophys. Res. Commun.18, 824 (1965).
Hamkalo B. A., Miller O. L.: Electron microscopy of genetic activity.Ann. Rev. Biochem.42, 379 (1973).
Hochmannová J.: Analysis of the intracellular DNA-protein associations inEscherichia coli andBacillus subtilis.Folia Microbiol.20, 103 (1975).
Ivarie R. D., Pène J. J.: Association of theBacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients.J. Bacteriol.104, 839 (1970).
Ivarie R. D., Pène J. J.: Association of many regions of theBacillus subtilis chromosome with the cell membrane.J. Bacteriol.114, 571 (1973).
Jacob F., Brenner S., Cuzin F.: On the regulation of DNA replication in bacteria.Gold Spring Harbor Symp. Quant. Biol.28, 329 (1963).
Kellenberger E., Ryter A., Séchaud J.: Electron microscope study of DNA-containing plasms. II. Vegetative and nature phage DNA as compared with normal bacterial nucleotide in different physiological states.J. Biophys. Biochem. Cytol.4, 671 (1958).
Kirby Berry H., Sutton H. E., Cain L., Berry J. S.: II. Development of paper chromatography for use in the study of metabolic patterns.Univ. Texas Pubs.5109, 22 (1951).
Konings W. N., Bisschop A., Veenhuis M., Vermeulen C. A.: New procedure for the isolation of membrane vesicles ofBacillus subtilis and an electron microscopy study of their ultrastructure.J. Bacteriol.116, 1456 (1973).
Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J.: Protein measurement with Folin phenol reagent.J. Biol. Chem.193, 265 (1951).
O’Sullivan M. A., Sueoka N.: Membrane attachment of the replication origins of a multifork (dichotomous) chromosome inBacillus subtilis.J. Mol. Biol.69, 237 (1972).
Reynolds E. S.: The use of lead citrate at high pH as an electronoptique stain in electron microscopy.J. Cell. Biol.17, 208 (1963).
Smith D. W., Hanawalt P. C.: Properties of the growing point region in the bacterial chromosome.Biochim. Biophys. Acta149, 519 (1967).
Snyder R. W., Young F. E.: Association between the chromosome and the cytoplasmic membrane inBacillus subtilis.Biochem. Biophys. Res. Commun.35, 354 (1969).
Sueoka N., Quinn W. G.: Membrane attachment of the chromosome replication origin inBacillus subtilis.Cold Spring Harbor Symp. Quant. Biol.33, 695 (1968).
Wake R. G.: Visualization of reinitiated chromosomes inBacillus subtilis.J. Mol. Biol.68, 501 (1972).
Yamaguchi K., Murakami S., Yoshikawa H.: Chromosome-membrane association inBacillus subtilis. I. DNA release from membrane fraction.Biochem. Biophys. Res. Commun.44, 1559 (1971).
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Hochmannová, J., Ludvík, J. Biochemical characterization and visualization of plasma membrane-DNA-protein complexes fromBacillus subtilis . Folia Microbiol 21, 117–124 (1976). https://doi.org/10.1007/BF02876978
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DOI: https://doi.org/10.1007/BF02876978