Abstract
The resistance of majority ofM. tuberculosis strains to rifampicin a key drug in the tuberculosis therapy, has been cause of major alarm world-wide. Such an association of strain with the drug takes a long time i.e. 2–3 months. We have modified a method for rapid detection of rifampicin resistant strains by screening them through PCR-SSCP.
We have identified mutations in rpo, β region of the gene encoding, β sub-unit of RNA polymerase in rifampicin resistant 67 clinical isolates ofM. tuberculosis. Mutations were screened in these isolates by single strand confirmation polymorphism (SSCP). When DNA sequencing data was compared with the result of SSCP analysis, direct PCR sequencing results were more easily interpreted and contatined more sequence dependent information. These findings provide the basis for rapid detection of rifampicin esistance, a marker of multidrug resistance.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Centre for disease control and prevention. (1993) Initial therapy for tuberculosis in the area of multidrug resistance: recommendations of the advisory council for the elimination of tuberculosis. Morbid. Mortal. Weekly Rep. 42, RR-703–704.
Centre for disease control. (1989) A strategic plan for the elimination of tuberculosis in the United States. Morbid. Mortality Weekly Report 38, (Suppl. 5-3) 1–25.
Freiden, T.R., Sterling, A., Pahlos-mendez, J.D., et al. (1993) The emergence of drug resistant tuberculosis in New York City. N. Eng. J. Med. 328, 521–526.
Telenti, A.P., Imboden, F., Marchese, D., et al. (1993) Detection of rifamicin resistance mutation inMycobacterium tuberculosis New Eng. J. Med. 341, 647–650.
Kapur, V., Lil, L., Lorddnescu, M.R., et al. (1995). Characterization of automated DNA sequenceing of mutations in the gene (rpoB) encoding the RNA polymerase of B subunit in rifampicin resistantMycobacterium tuberculosis strains from New York City and Texas. J. Clin, Micro. 32, 1095–1098.
Jaber, M., Rattan, A., Verma, A., et. al. (1995) A simple method of DNA extraction fromMycobacterium tuberculosis, 76, 578–581.
Felmlee, T.A., Liu A.Q., Whelen, C., et. al. (1995) Genotypic detection ofMycobacterium tuberculosis rifampicin resistance: comparison of single strand confirmation polymorphism and dideoxy fingerprinting. J. Clin. Microbiology, 33, 1617–1623.
Siddiqi, S., Libonati, J. and Middlebrook (1981) Evaluation of a rapid method for drug susceptibility testing ofMycobacterium tuberculosis. J. Clin. Micro, 13, 908–912.
Khanna, M., Goel A., Jain, R.C. and Srivastave, L.M. (1998) Rapid and a simple method of identification of rifampicin resistant strains ofM. tuberculosis. Communicated to Ind. J. Exptl. Biol.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Srivastava, L.M., Khanna, M., Goel, A. et al. Genotypic detection of mutations in rifampicin resistant clinical isolates ofMycobacterium tuberculosis . Indian J Clin Biochem 12 (Suppl 1), 83–85 (1997). https://doi.org/10.1007/BF02873069
Issue Date:
DOI: https://doi.org/10.1007/BF02873069