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Lytic effect ofVibrio cholerae elastase on Gram-positive and-negative bacteria

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Abstract

Elastase ofVibrio cholerae caused the lysis of freshly grown cells of Gram-negative (Pseudomonas aeruginosa, Proteus vulgaris, Salmonella paratyphi A andKlebsiella pneumoniae) bacteria. Gram-positive (Staphylococcus aureus andS. epidermidis) organisms were resistant to this enzyme. Heat killed and lyophilized Gram-positive and-negative bacteria (exceptS. aureus andS. epidermidis) showed higher sensitivity to elastase. Both Gram-negative and-positive bacteria were lyzed maximally by elastase at pH 8.0. At this pH, lytic activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers.

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Authors and Affiliations

  1. Department of Microbiology, Panjab University, 160 014, Chandigarh, India

    M. Kaur, M. Gupta & K. G. Gupta

  2. Central Research Institute, Kasauli, India

    K. K. Tripathi

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  1. M. Kaur
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  2. M. Gupta
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  3. K. K. Tripathi
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  4. K. G. Gupta
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Kaur, M., Gupta, M., Tripathi, K.K. et al. Lytic effect ofVibrio cholerae elastase on Gram-positive and-negative bacteria. Folia Microbiol 35, 183–189 (1990). https://doi.org/10.1007/BF02820483

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  • DOI: https://doi.org/10.1007/BF02820483

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