Abstract
Cytochalasin D, an inhibitor of actin polymerization, interferes with ascogonial differentiation in the fertilefluffy mutant ofNeurospora crassa. As the total level of actin and its mRNA remain unchanged, this suggests that it is in its microfilamentous form (F-form) that actin is stringently required for female differentiation. *** DIRECT SUPPORT *** A00EN042 00011
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Baria F., Coughlin C., Belin D., Gabbiani G.: Actin isoform synthesis and mRNA levels in quiescent and proliferating rat aortic smooth muscle cellsin vivo andin vitro.Lab. Invest. 55, 226–233 (1986).
Barja F., Nguyen Thi B.N. Turian G.: Localization of actin and characterization of its isoforms in the hyphæ ofNeurospora crassa FEMS Microbiol.Lett. 77, 19–24 (1991).
Burnette W.N.: Western blotting: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and ratio-iodinated protein A.Anal. Biochem. 112, 195–203 (1981).
Chirgwin J.M., Przybyla A.E., MacDonald R.J., Rumer W.J.: Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.Biochemistry 18, 5294–5299 (1979).
Laemmli U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T.Nature 227, 680–685 (1970).
Nguyen Thi B.N., Turian G.: Actin stringently accumulated in the specifically-positioned, differentiating female gametangia ofAllomyces.Sex. Plant Reprod. 5, 310–312 (1992).
Nguyen Thi B.N., Turian G.: Comparative inhibitory effects of cytochalasins on the gametangial differentiation inAllomyces arbuscula.Folia Microbiol. 38, 205–208 (1993).
Perkins D.D., Radford A., Newmeyer D., Björkman M.: Chromosomal loci ofNeurospora crassa.Microbiol. Rev. 46, 426–570 (1982).
Spector T.: Refinement of the Coomasie blue method of protein quantitation.Anal. Biochem. 86, 142–146 (1978).
Sampath P., Pollard T.D.: Effects of cytochalasin, phalloidin, and pH on the elongation of actin filaments.Biochemistry 30, 1973–1980 (1991).
Tannenbaum J.: Approaches to the molecular biology of cytochalasin action, pp. 521–540 inCytochalasins Biochemical and Cell Biological Aspects (S.W. Tannenbaum, Ed.). North-Holland, Amsterdam 1978.
Turian G., Bianchi D.E.: Conidiation inNeurospora.Bot. Rev. 38, 119–154 (1972).
Viswanath-Reddy M., Turian G.: Physiological changes during protoperithecial differentiation inNeurospora tetrasperma.Physiol. Plant. 35, 166–174 (1975).
Wuethrich G., Maeder B., Bolivar I., Barja F., Turian G.: Cloning of the actin gene ofNeurospora crassa.Experientia 50, A84 (1994).
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Liu, J.W., Turian, G. & Barja, F. A stringent role of F-actin in the ascogonial differentiation ofNeurospora crassa Fluffy mutant. Folia Microbiol 40, 665–668 (1995). https://doi.org/10.1007/BF02818527
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DOI: https://doi.org/10.1007/BF02818527