Skip to main content
SpringerLink
Log in

Effect of the carbon source on assessment of degrading bacteria with the spread-plating technique duringin situ bioremediation

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Spread-plating belongs to traditional microbiological methods employed for quantification of subsurface microflora during bioremediation projects in the Czechia. Concentration of degrading organisms is estimated from the number of colonies grown on agar plates supplied with contaminant as the sole carbon source. The data obtained duringin situ bioremediation of the Dačice site contaminated by cutting oil suggests that changes in the composition of the carbon source in the subsurface may cause a discrepancy between laboratory data and situation in subsurface.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DNAPL:

dense nonaqueous-phase liquid

LNAPL:

less dense nonaqueous-phase liquid

DAPI:

4′, 6-diamidino-2-phenylindole dihydrochloride

References

  • van Belkum A., Hermans P.W.M., Licciardello L., Stefani S., Grubb W., van Leeuwen W., Goessens W.H.F.: Polymerase chain reaction-mediated typing of microorganisms: tracking dissemination of genes and genomes—minireview.Electrophoresis 19, 602–607 (1998).

    Article  PubMed  Google Scholar 

  • Cookson J.T.:Bioremediation Engineering. McGraw-Hill, New York 1995.

    Google Scholar 

  • Damborský J.: Tetrachloroethene-dehalogenating bacteria.Folia Microbiol. 44, 247–262 (1999).

    Article  Google Scholar 

  • van Elsas J.D., Duarte G.F., Rosado A.S., Smalla K.: Microbiological and molecular biological methods for monitoring microbial inoculants and their effects in the soil environment.J. Microbiol. Meth. 32, 133–154 (1998).

    Article  Google Scholar 

  • Heitzer A., Sayler G.S.: Monitoring the efficacy of bioremediation.Trends Biotech. 11, 334–343 (1993).

    Article  CAS  Google Scholar 

  • Hohener P., Hunkeler D., Hess A., Bregnard T., Zeyer J.: Methodology for the evaluation of engineeredin situ bioremediation: lessons from a case study.J. Microbiol. Meth. 32, 179–192 (1998).

    Article  CAS  Google Scholar 

  • Matthews J.E. (Ed.)Handbook, of Bioremediation Lewis Publishers, Boca Raton 1994.

    Google Scholar 

  • Power M., van der Meer J.R., Tchelet R., Egli T., Eggen R.: Molecular-based methods can contribute to assessments of toxicological risks and bioremediation strategies.J. Microbiol. Meth. 32, 107–119 (1998).

    Article  CAS  Google Scholar 

  • Rojas-Avelizapa N.G., Rodríguez-Vázquez R., Martínez-Cruz J., Esparza-García F., Montes De Oca-García A., Ríos-Leal E., Fernández-Villagómez G.: Isolation and characterization of bacteria degrading polychlorinated biphenyls from transformer oil.Folia Microbiol. 44, 317–322 (1999).

    CAS  Google Scholar 

  • Sayler G.S., Layton A., Lajoie C., Bowman J., Tschantz M., Fleming J.T.: Molecular site assessment and process monitoring in bioremediation and natural attenuation.Appl. Biochem. Biotech. 54, 277–290 (1995).

    Article  CAS  Google Scholar 

  • Stapleton R.D., Ripp S., Jimenez L., CheolKoh S., Fleming J.T., Gregory I.R., Sayler G.S.: Nucleic acid analytical approaches in bioremediation: site assessment and characterization.J. Microbiol. Meth. 32, 165–178 (1998).

    Article  CAS  Google Scholar 

  • Suthersan S.S.:Remediation Engineering. CRC Press, Boca Raton 1997.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Biomolecular Structure and Dynamics, Faculty of Science, Masaryk University, 611 37, Brno, Czechia

    J. Damborský, L. Trantírek & V. Sklenár

  2. Inpost Ltd., 686 01, Uherské Hradiště, Czechia

    M. Damborská & S. Štípek

  3. Department of Environmental Chemistry and Ecotoxicology, Faculty of Science, Masaryk University, 611 37, Brno, Czechia

    A. Jesenská

Authors
  1. J. Damborský
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Damborská
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Štípek
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Jesenská
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. Trantírek
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. Sklenár
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Damborský.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Damborský, J., Damborská, M., Štípek, S. et al. Effect of the carbon source on assessment of degrading bacteria with the spread-plating technique duringin situ bioremediation. Folia Microbiol 45, 35–40 (2000). https://doi.org/10.1007/BF02817447

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02817447

Keywords

Search

Navigation