Abstract
The interaction of liposomes loaded with Ponceau red (used as a marker) withPseudomonas aeruginosa cells was observed and it resulted in marker leakage. The marker leakage from liposomes was low in physiological fluids. The interaction was independent of secreted phospholipase C level and the serotype of the tested strain. Six of 37 examined isolates did not cause any release of the marker from the liposomes. Marker release of over 50% of total encapsulated material was observed only for ten of the strains tested.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Betageri G.V., Parsons D.L.: Drug encapsulation and release from multilamellar and unilamellar liposomes.Int. J. Pharmaceut. 81, 235–241 (1992).
Esselmann M.T., Liu P.V.: Lecithinase production by gram-negative bacteria.J. Bacteriol. 81, 939–945 (1961).
Goñi F.M., Nieva J.L., Basanez G., Fidelio G., Alonso A.: Phospholipase C promoted liposome fusion.Biochem. Soc. Trans. 22, 839–844 (1994).
Lagace J., Dubreuil M., Montplaisi R.S.: Liposome-encapsulated antibiotics: preparation, drug release and antimicrobial activity againstPseudomonas aeruginosa.J. Microencapsulation 8, 53–61 (1991).
Naccuchio M.C., Bellora M.J.G., Sordelli D.O., D'Aquino M.: Enhanced liposome-mediated antibacterial activity of piperacillin and gentamicin against gram-negative bacilliin vitro.J. Microencapsulation 5, 303–309 (1988).
New R.R.C.: Preparation of liposomes, pp. 30–103 in R.R.C. New (Ed.):Liposomes. A Practical Approach, Oxford University Press, Oxford 1990.
New R.R.C., Black C.D.V., Parker R.J., Puri A., Scherphof G.L.: Liposomes in biological systems, pp. 221–253 in R.R.C. New (Ed.):Liposomes. A Practical Approach. Oxford University Press, Oxford 1990.
Nicholov R., Khoury A.E., Bruce A.W., Dicosmo F.: Interaction of ciprofloxacin loaded liposomes withPseudomonas aeruginosa cells.Cells & Materials 3, 321–326 (1993).
Rostand K.S., Esko J.D.: Cholesterol and cholesterol esters: Host receptors forPseudomonas aeruginosa adherence.J. Biol. Chem. 268, 24053–24059 (1993).
Stinson M.W., Hayden C.: Secretion of phospholipase C byPseudomonas aeruginosa.Infect. Immun. 25, 558–564 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Antos, M., Trafny, E.A. & Grzybowski, J. The influence ofPseudomonas aeruginosa on liposomes. Folia Microbiol 41, 29–32 (1996). https://doi.org/10.1007/BF02816336
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02816336