Skip to main content
Log in

A rapid and simple method for DNA preparation fromCandida utilis

  • Brief Report
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

A method for the extraction of genomic DNA from the industrial yeastCandida utilis is described. The method is rapid, simple and produces DNA that is sufficiently pure for restriction analysis and should be suitable for Southern blotting and the construction of gene libraries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Cryer D.R., Eccleshall R., Marmur J.: Isolation of yeast DNA.Methods Cell Biol. 12, 39–44 (1975).

    PubMed  CAS  Google Scholar 

  • Davis R.W., Thomas M., Cameron J.R., John T.P.S., Sherer S., Padgett R.A.: Rapid DNA isolation for enzymatic and hybridization analysis.Methods Enzymol. 65, 404–411 (1980).

    Article  PubMed  CAS  Google Scholar 

  • Glee P.M., Russel P.J., Welsch J.A., Pratt J.C., Cutler J.E.: Methods for DNA extraction fromCandida albicans.Anal. Biochem. 164, 207–213 (1987).

    Article  PubMed  CAS  Google Scholar 

  • Holm C., Meeks-Wagner D.W., Fangman W.L., Botstein D.: A rapid, efficient method for isolating DNA from yeast.Gene 42, 169–173 (1986).

    Article  PubMed  CAS  Google Scholar 

  • Rothstein R.: Isolation of yeast DNA, p. 56 inDNA Cloning, a Practical Approach, Vol. II (D. M. Glover Ed.). IRL Press Ltd., Oxford-Washington (DC) 1985.

    Google Scholar 

  • Sherman F., Fink G.R., Hicks J.B.: Yeast DNA preparation, p. 77 inMethods in Yeast Genetics, A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor (New York) 1983.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Križková, L., Jeloková, J. A rapid and simple method for DNA preparation fromCandida utilis . Folia Microbiol 36, 406–407 (1991). https://doi.org/10.1007/BF02814518

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02814518

Keywords

Navigation