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Sensitivity of cultured pancreatic carcinoma cells toAcinetobacter glutaminase-asparaginase

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Summary

Cultured human pancreatic carcinoma cells (MIA PaCa-2) have been shown previously to be very sensitive toE. coli l-asparaginase (EC II). The present studies have demonstrated that another enzyme,Acinetobacter glutaminase-asparaginase (AGA) is much more effective in inhibiting cell growth. At the concentration of 0.0025 U/ml of AGA activity the enzyme totally inhibited cell growth, whereas the EC II with the same concentration did not show any effect. The inhibition of cell growth correlated well with inhibition of protein and glycoprotein synthesis. The addition ofl-glutamine at the concentration of 1 mM completely reversed the inhibition of protein synthesis. Similarly, the addition ofl-glutamine at the concentration of 3 mM daily on 3 successive days after adding AGA resulted in significant reversal of growth inhibition. The results of this study indicate that the action of AGA on MIA PaCa-2 is, to a great extent, exerted through itsl-glutaminase activity.

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Authors and Affiliations

  1. Department of Pediatrics, The Medical College of Wisconsin, 53233, Milwaukee, Wisconsin

    John S. Holcenberg

  2. Department of Medicine (R-38), University of Miami School of Medicine, P.O. Box 016960, 33101, Miami, Florida

    Ming-Chi Wu, Grace K. Arimura & Adel A. Yunis

Authors
  1. Ming-Chi Wu
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  2. Grace K. Arimura
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  3. John S. Holcenberg
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  4. Adel A. Yunis
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Additional information

This work was supported in part by USPHS Grant CA 19182. Dr. Wu is recipient of Research Career Development Award Grant CA00686 and Dr. Yunis is a Howard Hughes Investigator.

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Wu, MC., Arimura, G.K., Holcenberg, J.S. et al. Sensitivity of cultured pancreatic carcinoma cells toAcinetobacter glutaminase-asparaginase. In Vitro 18, 750–754 (1982). https://doi.org/10.1007/BF02796498

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  • DOI: https://doi.org/10.1007/BF02796498

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