Skip to main content
SpringerLink
Log in

Analysis of biomass cellulose in simultaneous saccharification and fermentation processes

  • Original Articles
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A direct method for determining the cellulose content of biomass residues resulting from simultaneous saccharifiaction and fermentation (SSF) experiment has been developed and evaluated. The method improves on classical cellulose assays by incorporating the enzymatic removal of yeast glucans from the biomass residue prior to acid hydrolysis and subsequent quantification of cellulose-derived glucose. An appropriate cellulasefree, commercially available, yeast-lysing enzyme preparation fromCytophaga was identified. A freeze-drying step was identified as necessary to render the SSF yeast cells susceptible to enzymatic lysis. The method was applied to the analysis of cellulose and yeast-associated glucans in SSF residues from three pretreated feedstocks; hybrid poplar, switchgrass, and cornstover. Cellulose assays employing the lysing-enzyme preparation demonstrated relative errors up to 7.2% when yeast-associated glucans were not removed prior to analysis of SSF residues. Enzymatic lysis of SSF yeast cells may be viewed as a general preparatory procedure to be used prior to subsequent chemical and physical analysis of SSF residues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Spindler, D. D., Wyman, C. E., and Grohmann, K. (1991),Appl. Biochem. Biotechnol. 28/29, 773–786.

    Google Scholar 

  2. Shan, M. M. and Lee, Y. Y. (1992),Appl. Biochem. Biotechnol. 34/35, 557–568.

    Google Scholar 

  3. Wyman, C. E., Spindler, D. D., and Grohmann, K. (1992),Biomass Bioenerg. 3, 301–307.

    Article  CAS  Google Scholar 

  4. Vinzant, T., Panfick, L., Nagle, N. J., Ehrman, C. I., Reynolds, J. B., and Himmel, M. E. (1994),Appl. Biochem. Biotechnol. 45/46, 611–626.

    Google Scholar 

  5. Spindler, D. D., Wyman, C. E., and Grohmann, K. (1989a),Biotechnol. Bioeng. 34, 189–195.

    Article  CAS  Google Scholar 

  6. Spindler, D. D., Wyman, C. E., Grohmann, K., and Mohagheghi, A. (1989b),Appl. Biochem. Biotechnol. 20/21, 529–540.

    Google Scholar 

  7. Mohagheghi, A., Tucker, M., Grohman, K., and Wyman, C. (1991),Appl. Biochem. Biotechnol. 33, 67–81.

    Article  Google Scholar 

  8. Meyers, S. G. (1978),Am. Inst. Chem. Eng. 184 (74), 79–84.

    Google Scholar 

  9. Spindler, D. D., Wyman, C. E., Mohagheghi, A., and Grohmann (1988),Appl. Biochem. Biotechnol. 16/17, 279–293.

    Article  Google Scholar 

  10. Kreutzfeldt, C. and Witt, C. C. (1991), inSaccharomyces, vol. Tuite, M. F., and Oliver, S. G., eds., Plenum Press, New York, NY, pp. 5–58.

    Google Scholar 

  11. Fleet, G. H. (1991), inThe Yeasts, vol. 4, Rose, A. H. and Harrison, J. S., eds, Academic Press, San Diego, CA, pp. 199–277.

    Google Scholar 

  12. Wyman, C. E., Spindler, D. D., Grohmann, K., and Lastick, S. M. (1986),Biotechnol. Bioeng. Symp. 17, 221–238.

    CAS  Google Scholar 

  13. Grethlein, H. E. and Converse, A. O. (1991),Bioresource Technol. 77–82.

  14. Fan, L. T., Lee, Y, H., and Beardmore, D. H. (1980), inProceeding of Bioconversion and Biochemical Engineering Symposium, vol. 1, Ghose, T. K., ed., Indian Institute of Technology, Hauzkhas, New Delhi, India, pp. 233–259.

    Google Scholar 

  15. Ghose, T. K. (1988),Pure Appl. Chem. 59, 257–268.

    Article  Google Scholar 

  16. Fenske, J. J. (1994), Master’s thesis, Oregon State University, Corvallis, Oregon.

    Google Scholar 

  17. Philippidis, G. P., Smith, T. K., and Schmidt, S. L. (1993), SSF experimental protocols: Lignocellulosic biomass hydrolysis and fermentation.

  18. Moore, W. E. and Johnson, D. B. (1967), Procedures for the chemical analysis of wood and wood products, USDA Forest Products Laboratory, Madison, WI.

    Google Scholar 

  19. Ehrman, C. I. and Himmel, M. E. (1994), Biotechnol. Tech.8, 99–104.

    Article  CAS  Google Scholar 

  20. Tuite, M. F. and Oliver, S. G. (1991), inSaccharomyces, Tuite, M. F. and Oliver, S. G., eds., Plenum Press, New York, NY, pp. 283–320.

    Google Scholar 

  21. Sigma Chemical Co. (1996),Product Information Catalog.

  22. Stainer, R. Y., Adelberg, E. A., and Ingraham, J. L. (1976),The Microbial World, 4th ed., Prentice-Hall, Inc., New Jersey.

    Google Scholar 

  23. Tatsumoto, K., Baker, J. O., Tucker, M. P., Oh, K. K., Mohaghehi, A., Grohmann, K., and Himmel, M. E. (1988),Appl. Biochem. Biotechnol. 18, 159–173.

    Article  CAS  Google Scholar 

  24. Suttcliffe, R. and Saddler, J. N. (1986),Biotechnol. Biogeng. Symp. 17, 749–762.

    Google Scholar 

  25. Sinitsy, A. P. Bungay, H. R. and Clesceri, L. S. (1983),Biotechnol. Bioeng.XXV, 1393–1399.

    Article  Google Scholar 

  26. Sommer, A. and Lewis, A. J. (1971),J. Gen Microb. 68, 327–335.

    Google Scholar 

  27. Souzu, H. (1973),Cryobiology 10, 427–431.

    Article  CAS  Google Scholar 

  28. Van Steveninck, J. and Ledeboer, A. M. (1974),Biochem. Biophys. Acta. 352, 64–70.

    Article  Google Scholar 

  29. Kruuv, J., Lopock, J. R., and Keith, A. D. (1978),Cryobiology. 15, 73–79.

    Article  CAS  Google Scholar 

  30. Eddy, A. A. and Williamson, D. H. (1957),Nature. 179, 1252–1253.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food Science and Technology, Oregon State University, 97331-6602, Corvallis, OR

    Yun-Chin Chung, Alan Bakalinsky & Michael H. Penner

Authors
  1. Yun-Chin Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alan Bakalinsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michael H. Penner
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Oregon State University Agricultural Experiment Station Technical Publication Number 10977.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chung, YC., Bakalinsky, A. & Penner, M.H. Analysis of biomass cellulose in simultaneous saccharification and fermentation processes. Appl Biochem Biotechnol 66, 249–262 (1997). https://doi.org/10.1007/BF02785591

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02785591

Index Entries

Search

Navigation