Abstract
Polyaldehyde microspheres, polyglutaraldehyde (PGL), and polyacrolein (PA) were synthesized by polymerizing glutaraldehyde and acrolein in the presence of an appropriate surfactant. The microspheres with average diameter of 0.2 μm were used for the specific labeling of human red blood cells (RBC) and mouse lymphocytes. The “naked” microspheres were encapsulated with agarose and formed agarose-polyaldehyde microsphere beads in sizes ranging from 50 μm up to 1 cm. The encapsulated beads, with diameters ranging from 50 to 150 μm were used as insoluble adsorbents for affinity purification of antibodies. Beads with diameters varied from 150 to 250 μm were used for cell fractionation purposes (mouse B splenocytes from T splenocytes). Uniform beads of 1 mm diameter were designed for hemoperfusion purposes. As a model, the removal in vitro of anti-BSA from immunized goat whole blood was studied.
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Margel, S. Agarose-polyaldehyde microsphere beads: Synthesis and biomedical applications. Appl Biochem Biotechnol 8, 523–539 (1983). https://doi.org/10.1007/BF02780385
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DOI: https://doi.org/10.1007/BF02780385
Index Entries
- Agarose-polyaldehyde microsphere beads
- polyaldehyde-agarose microsphere beads
- microspheres, agarose-polyaldehyde
- synthesis, of agarose-polyaldehyde microspheres
- cell labeling, with agarose-polyaldehyde microspheres
- cell separation, with agarose-polyaldehyde microspheres
- affinity chromatography, with agarose-polyaldehyde microspheres
- hemoperfusion, with agarose-polyaldehyde microsphere
- chromatography, affinity with agarose-polyaldehyde microspheres