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Lipid peroxidation and lysosomal enzymes in D-galactosamine hepatitis and its protection by vitamin E

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Summary

Role of Iipid peroxidation on lysosomal instability in liver tissue was investigated in an experimental model of D-galactosamine hepatitis in rats fed on vitamin E (V.E) deficient diet.

Administration of D-galactisamine to V.E deficient rats resulted in a sudden increase of serum glutamic oxaloacetic transaminase (sGOT), glutamic pyruvic transaminase (sGPT), lipid peroxide value, as well asβ glucuronidase and acid phosphatase activity examined as markers of lysosomal enzymes, when compared with control rats fed on V.E supplemented diet.

Lipid peroxide in the liver tissue also showed significant increase in V.E deficient rats. In contrast, \- glucuronidase and acid phosphatase in the liver tissue were found to decrease in V.E deficient rats by the administration of D-galactosamine, indicating that the enzymes in the lysosome were entirely released outside the liver cells as a result of cell destruction.

It is concluded that the increase of lipid peroxide causes the instability of lysosomal membranes and releases various kinds of hydrolytic enzymes to lead further cell damage. V.E might act on inhibiting lipid peroxidation to stabilize lysosomal membranes.

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Authors and Affiliations

  1. The First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, 602, Kyoto, Japan

    Toshikazu Yoshikawa M.D. & Motoharu Kondo M.D.

  2. The Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto

    Nobuyoshi Yokoe M.D., Shuhei Takemura M.D., Haruki Kato M.D., Tadahiro Hotta M.D., Naoyuki Matsumura M.D., Minoru Ikezaki M.D. & Keimei Hosokawa M.D.

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  1. Toshikazu Yoshikawa M.D.
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  2. Nobuyoshi Yokoe M.D.
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  3. Shuhei Takemura M.D.
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  4. Haruki Kato M.D.
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  5. Tadahiro Hotta M.D.
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  6. Naoyuki Matsumura M.D.
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  7. Minoru Ikezaki M.D.
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  8. Keimei Hosokawa M.D.
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  9. Motoharu Kondo M.D.
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Yoshikawa, T., Yokoe, N., Takemura, S. et al. Lipid peroxidation and lysosomal enzymes in D-galactosamine hepatitis and its protection by vitamin E. Gastroenterol Jpn 14, 31–39 (1979). https://doi.org/10.1007/BF02774602

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  • DOI: https://doi.org/10.1007/BF02774602

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