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Effects of adenine arabinoside on cellular immune responses in patients with chronic hepatitis B

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Summary

The lymphocyte subsets in the peripheral blood and in liver biopsies from 4 patients with chronic hepatitis B obtained about 2–7 weeks before and after treatment with adenine arabinoside (Ara-A) were studied by a peroxidase-labeled antibody method using monoclonal antibodies against Leu-1, Leu-2a, Leu-3a, Leu-7 and Leu-10 antigens. In the peripheral blood, the percentage of Leu-2a + (cytotoxic/suppressor) cells was significantly reduced and the ratio of Leu-3a + (helper/inducer) to Leu-2a + cells was increased after the treatment with Ara-A. In the liver biopsies, the numbers of Leu-1 + (pan T) and Leu-2a + cells were significantly decreased after the treatment with Ara-A. As a result, the Leu-3a + /Leu-2a + ratio was significantly elevated in the liver after the therapy. The majority of lymphocytes distributed at sites of hepatocytic necrosis were positive for Leu-2a. The reduced numbers of Leu-1 + and Leu-2a + cells after the treatment were mainly due to the decrease of these cells infiltrating to the sites of hepatocytic necrosis. The numbers of other subsets (Leu-3a +, Leu-7 + and Leu-10 +) changed without any specific tendency both in the peripheral blood and in the liver biopsies after the treatment. With respect to viral replication, most of the patients showed a decrease of serum DNA polymerase activity or demonstrable intrahepatic HBsAg and HBcAg after the treatment. These data suggest that T cell-mediated cytotoxicity against HBV-infected hepatocytes is diminished after treatment with Ara-A.

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Authors and Affiliations

  1. The First Department of Internal Medicine, Okayama University Medical School, 2-5-1, Shikata-cho, 700, Okayama, Japan

    Ichinosuke Hyodo M.D., Gotaro Yamada M.D., Koji Manabe M.D., Hiroaki Okushin M.D., Motowo Mizuno M.D. & Hideo Nagashima M.D.

Authors
  1. Ichinosuke Hyodo M.D.
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  2. Gotaro Yamada M.D.
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  3. Koji Manabe M.D.
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  4. Hiroaki Okushin M.D.
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  5. Motowo Mizuno M.D.
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  6. Hideo Nagashima M.D.
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Additional information

This study was supported in part by grants from the Japanese Ministry of Health and Welfare, and from the Japanese Ministry of Education (Grant No. 57570280).

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Hyodo, I., Yamada, G., Manabe, K. et al. Effects of adenine arabinoside on cellular immune responses in patients with chronic hepatitis B. Gastroenterol Jpn 21, 220–227 (1986). https://doi.org/10.1007/BF02774564

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  • DOI: https://doi.org/10.1007/BF02774564

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