Skip to main content
SpringerLink
Log in

New approaches to lifetime-resolved luminescence imaging

  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

An overview of recent developments in time-resolved imaging technology is presented. A directly modulated CCD imaging device has been developed which is suitable for frequency-domain phosphorescence measurements up to more than 400 kHz. This has been used with a recently-developed blue/UV LED source to implement a low-noise, solid-state phosphorescence/fluorescence imaging system suitable for macroscopic and microscopic imaging. For shorter lifetimes a range of new light sources has become available with higher light output and/or higher frequency modulation performance. Acoustooptic tunable filters have also been investigated as optical “light gates” both for modulation of excitation and for time-resolved imaging, and the limits of performance of these devices are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. L. M. Wang, P. P. Ho, and R. R. Alfano (1993)Appl. Opt. 32(4), 535–540.

    Article  Google Scholar 

  2. C. G. Morgan, A. C. Mitchell, N. Peacock, and J. G. Murray (1995)Rev. Sci. Instrumen. 66(1), Pt 1, 48–52.

    Article  CAS  Google Scholar 

  3. C. G. Morgan, A. C. Mitchell, and J. G. Murray (1992)J. Microsc. 165(1), 49–60.

    Google Scholar 

  4. C. G. Morgan, Y. Hua, A. C. Mitchell, J. G. Murray, and A. D. Boardman (1996)Rev. Sci. Instrumen. 67(1), 41–47.

    Article  CAS  Google Scholar 

  5. T. Araki and H. Misawa (1995)Rev. Sci. Instrumen. 66(12), 5469–5472.

    Article  CAS  Google Scholar 

  6. H. R. Morris, C. C. Hoyt, and P. J. Treado (1994)Appl. Spectrosc. 48(7), 857–866.

    Article  CAS  Google Scholar 

  7. J. R. Lakowicz (1983)Principles of Fluorescence Spectroscopy, Plenum Press, New York.

    Google Scholar 

  8. R. K. Reich, R. W. Mountain, W. H. McGonagle, C. M. Huang, J. C. Twitchell, B. B. Kosicki, and E. D. Savoye (1993)IEEE Trans. Electron. Dev. 40(7), 1231–1237.

    Article  Google Scholar 

  9. O. S. Wolfbeis, M. J. P. Leiner, and H. E. Posch (1986)Mikrochim. Acta 3, 359–366.

    Article  CAS  Google Scholar 

  10. J. C. Fister, D. Rank, and J. M. Harris (1995)Proc. Anal. Chem. 67(23), 4269–4275.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biophysics Group, Department of Biological Sciences, Science Research Institute, University of Salford, M5 4WT, Salford, UK

    C. G. Morgan, A. C. Mitchell, J. G. Murray & E. J. Wall

Authors
  1. C. G. Morgan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. C. Mitchell
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. G. Murray
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. J. Wall
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Morgan, C.G., Mitchell, A.C., Murray, J.G. et al. New approaches to lifetime-resolved luminescence imaging. J Fluoresc 7, 65–73 (1997). https://doi.org/10.1007/BF02764579

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02764579

Key Words

Search

Navigation