Abstract
The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.
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Abbreviations
- ConA:
-
Concanavalin A
- IPTG:
-
isopropyl-Β-D-thiogalactopyranoside
- PMSF:
-
phenyl methyl sulphonyl flouride
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Dincturk, H.B., Dunn, A.J. & Jones, D.H. Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility. J. Biosci. 26, 635–640 (2001). https://doi.org/10.1007/BF02704761
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DOI: https://doi.org/10.1007/BF02704761