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Cell volume changes affect gluconeogenesis in the perfused liver of the catfishClarias batrachus

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Abstract

In addition to lactate and pyruvate, some amino acids were found to serve as potential gluconeogenic substrates in the perfused liver ofClarias batrachus. Glutamate was found to be the most effective substrate, followed by lactate, pyruvate, serine, ornithine, proline, glutamine, glycine, and aspartate. Four gluconeogenic enzymes, namely phosphoenolpyruvate carboxykinase (PEPCK), pyruvate carboxylase (PC), fructose 1,6-bisphosphatase (FBPase) and glucose 6-phosphatase (G6Pase) could be detected mainly in liver and kidney, suggesting that the latter are the two major organs responsible for gluconeogenic activity in this fish. Hypo-osmotically induced cell swelling caused a significant decrease of gluconeogenic efflux accompanied with significant decrease of activities of PEPCK, FBPase and G6Pase enzymes in the perfused liver. Opposing effects were seen in response to hyperosmotically induced cell shrinkage. These changes were partly blocked in the presence of cycloheximide, suggesting that the aniso-osmotic regulations of gluconeogenesis possibly occurs through an inverse regulation of enzyme proteins and/or a regulatory protein synthesis in this catfish. In conclusion, gluconeogenesis appears to play a vital role inC. batrachus in maintaining glucose homeostasis, which is influenced by cell volume changes possibly for proper energy supply under osmotic stress.

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Abbreviations

FBPase:

Fructose 1,6-bisphosphatase

GDH:

glutamate dehydrogenase

GPase:

glycogen phosphorylase

G6Pase:

glucose-6-phosphatase

G6PDH:

glucose-6-phosphate dehydrogenase

GSase:

glycogen synthase

LDH:

lactate dehydrogenase

PC:

pyruvate carboxylase

PEPCK:

phosphoenol-pyruvate carboxykinase

RVD:

regulatory volume decrease

RVI:

regulatory volume increase

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Authors and Affiliations

  1. Biochemical Adaptation Laboratory, Department of Zoology, North-Eastern Hill University, 793 022, Shillong, India

    Carina Goswami, Shritapa Datta, Kuheli Biswas & Nirmalendu Saha

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  1. Carina Goswami
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  2. Shritapa Datta
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  3. Kuheli Biswas
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  4. Nirmalendu Saha
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Correspondence to Nirmalendu Saha.

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Goswami, C., Datta, S., Biswas, K. et al. Cell volume changes affect gluconeogenesis in the perfused liver of the catfishClarias batrachus . J Biosci 29, 337–347 (2004). https://doi.org/10.1007/BF02702616

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  • DOI: https://doi.org/10.1007/BF02702616

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