Abstract
The need for effective methods of cryopreservation of early mammalian embryos necessitates the study of the mechanisms of blastomere adaptation to the equilibration procedure and subsequent washing from the cryoprotector. The osmotic effects during these procedures can cause electrolyte imbalance in embryonic cells. Intracellular potassium concentrations at the stage of two-blastomere mouse embryo were studied by electron probe microanalysis.
Similar content being viewed by others
References
A.S. Krivokharchenko, L.I. Vil’yanovich, and G.A. Serobyan,Probl. Reproduktsii,4, 13–17 (1995).
E. I. Smol’yaninova, O.B. Khromenkova, and G.V. Zhernoklev,Probl. Kriobiol.,2, 49–55 (2001).
A. Pogorelov, B. Allachverdov, I. Burovina,et al., J. Microsc.,162, Pt. 2, 255–269 (1991).
A. Pogorelov, V. Pogorelova, N. Repin, and I. Mizin,Scanning Microsc. Suppl.,8, 101–108 (1994).
L. Wilson and P. Quinn,Hum. Reprod.,4, 86–90 (1989).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated fromByulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 138, No. 7, pp. 48–49, July, 2004
Rights and permissions
About this article
Cite this article
Gol’dshtein, D.V., Smol’yaninova, E.I. & Pogorelov, A.G. Potassium assay in blastomere of two-cell mouse embryo after equilibration and washing from cryoprotector. Bull Exp Biol Med 138, 40–41 (2004). https://doi.org/10.1007/BF02694469
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02694469