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A cell line selected for resistance to ionizing radiation exhibits cross resistance to other genotoxic agents and a mutator phenotype for loss of heterozygosity events

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Somatic Cell and Molecular Genetics

Abstract

An ionizing radiation resistant derivative was obtained from the mouse P19H22 (aprt hemizygote) embryonal carcinoma cell line by repeated exposure to137Cs gamma radiation. Ionizing radiation resistance in the 6Gy-R cell line was not correlated with a failure to undergo cell cycle arrest or a loss of the p53 response after exposure to137Cs gamma radiation. Moreover, the cells did not display increased resistance to bleomycin, a double strand break inducing agent. However, the cells did display increased resistance to ultraviolet radiation, ethyl methanesulfonate, and 95% oxygen. A mutational analysis demonstrated a>700 fold-fold increase in the frequency ofaprt mutants for the 6Gy-R cells, but no change in the frequency ofhprt ordhfr mutants. A molecular analysis suggested that theaprt mutations in the 6Gy-R cells arose by recombinational events. A possible association between radiation resistance, DNA repair, and a mutator phenotype for large-scale mutational events is discussed.

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Author notes

  1. Kimberly A. Walker

    Present address: Department of Biology, State University of New York at Albany, 12222, Albany, New York

  2. Mitchell S. Turker

    Present address: Center for Research on Occupational and Environmental Toxicology, L606, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, 97201, Portland, Oregon

Authors and Affiliations

  1. Department of Pathology, University of Kentucky, 40536, Lexington, Kentucky

    Kimberly A. Walker, C. Darrell Jennings, Joseph Pulliam & Mitchell S. Turker

  2. Department of Pathology, University of Washington, 98195, Seattle, Washington

    Charles Ogburn & George M. Martin

  3. Department of Radiation Medicine, University of Kentucky, 40536, Lexington, Kentucky

    Muneyaso Urano

  4. Markey Cancer Center, University of Kentucky, 40536, Lexington, Kentucky

    Mitchell S. Turker

Authors
  1. Kimberly A. Walker
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  2. C. Darrell Jennings
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  3. Joseph Pulliam
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  4. Charles Ogburn
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  5. George M. Martin
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  6. Muneyaso Urano
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  7. Mitchell S. Turker
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Walker, K.A., Jennings, C.D., Pulliam, J. et al. A cell line selected for resistance to ionizing radiation exhibits cross resistance to other genotoxic agents and a mutator phenotype for loss of heterozygosity events. Somat Cell Mol Genet 23, 111–121 (1997). https://doi.org/10.1007/BF02679970

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  • DOI: https://doi.org/10.1007/BF02679970

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