Summary
The changes in cell size and total protein were determined for G1-arrested, contact-inhibited CV-1 cells infected with Simian virus 40 (SV40). The assays used were the Biorad total protein assays (Bradford and DC protein assays) on a standard number of cells, total protein as assayed by fluorescein isothiocyanate (FITC) and SR101 by flow cytometry, orthoganol (90°) light scatter by flow cytometry, and direct microscopic measurement with an ocular micrometer. Uninfected CV-1 cells and two cell lines with variations in DNA content (diploid vs. tetraploid) were used as controls for the studies presented. The results demonstrated a 40–60% increase in total protein at 32 to 42 h postinfection. These increases were similar to values obtained as control cells progress through the cell cycle. At later times postinfection (>42 h), total protein decreased due to cellular changes resulting from viral replication and cell death.
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Lehman, J.M., Dickerson, E., Friedrich, T. et al. Increase in total protein following infection of CV-1 cells with SV40 virus as assayed by flow cytometry. In Vitro Cell Dev Biol - Animal 31, 806–810 (1995). https://doi.org/10.1007/BF02634124
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DOI: https://doi.org/10.1007/BF02634124