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Inositolhexakisphosphate (InsP6): An antagonist of fibroblast growth factor receptor binding and activity

  • Growth, Differentiation, And Senescence
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Summary

Fibroblast growth factors (FGF), which have been implicated in tumor cell growth and angiogenesis, have biological activities that appear to be mediated by both heparinlike extracellular matrix sites and transmembrane tyrosine kinase receptor sites. In the present study, we demonstrated that inositolhexakisphosphate (InsP6) inhibits basic FGF (bFGF) binding to heparin. Our spectrofluorometric analyses demonstrated that InsP6 not only bound to bFGF, presumably within the bFGF heparin-binding domain, but also protected bFGF from degradation by trypsin. Also, InsP6 inhibited the cellular binding of bFGF and other fibroblast growth factor family members such as acidic FGF (aFGF) and K-FGF in a saturable and dose-dependent manner. Furthermore, concentrations as low as 100µM InsP6 inhibited bFGF-induced DNA synthesis in AKR-2B fibroblasts, as well as the growth of bFGF-and K-FGF-transfected NIH/3T3 cells. Together, these results indicate that InsP6 may serve as a useful antagonist of FGF activity.

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Author notes

  1. Richard S. Morrison

    Present address: Department of Neurosurgery, RI-20, University of Washington School of Medicine, 1959 N.E. Pacific Street, 98195, Seattle, Washington

  2. Wallace McKeehan

    Present address: Institute of Bioscience and Technology, Texas A&M University, 77030, Houston, Texas

Authors and Affiliations

  1. Departments of Neurosurgery, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, 77030, Houston, Texas

    Richard S. Morrison & Fumio Yamaguchi

  2. Tumor Biology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, 77030, Houston, Texas

    Richard S. Morrison

  3. W. Alton Jones Cell Science Center, 10 Old Barn Road, 12946, Lake Placid, New York

    Ergang Shi, Mikio Kan & Wallace McKeehan

  4. Department of Ophthalmology, RJ-10, University of Washington School of Medicine, 1959 N.E. Pacific Street, 98195, Seattle, Washington

    Maria Rudnicka-Nawrot & Krzysztof Palczewski

Authors
  1. Richard S. Morrison
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  2. Ergang Shi
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  3. Mikio Kan
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  4. Fumio Yamaguchi
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  5. Wallace McKeehan
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  6. Maria Rudnicka-Nawrot
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  7. Krzysztof Palczewski
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Morrison, R.S., Shi, E., Kan, M. et al. Inositolhexakisphosphate (InsP6): An antagonist of fibroblast growth factor receptor binding and activity. In Vitro Cell Dev Biol - Animal 30, 783–789 (1994). https://doi.org/10.1007/BF02631302

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  • DOI: https://doi.org/10.1007/BF02631302

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