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Clonogenic assay of leukemic cells in serum-free culture

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References

  1. Casadevall, N.; Vainchenker, W.; Lacombe, C., et al. Erythroid progenitors in polychthemia vera: demonstration of their hypersensitivity to erythropoietin using serum-free cultures. Blood 59:447–451; 1982.

    PubMed  CAS  Google Scholar 

  2. Dai Y. C.; Hu, G. Z.; Niu, H. Y., et al. Formation of lymphocyte colonies under serum-free culture conditions in normal individuals and patients with chronic lymphocytic leukemia. Int. J. Cell Cloning 5:480–491; 1987.

    Article  PubMed  CAS  Google Scholar 

  3. Eliason, J. F.; Odartchenko, N. Colony formation by primitive hemopoietic progenitor cells in serum-free medium. Proc. Natl. Acad. Sci. USA 82:775–779; 1985.

    Article  PubMed  CAS  Google Scholar 

  4. Iscove, N. N.; Melchers, F. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B-lymphocytes. J. Exp. Med. 147:923–933; 1978.

    Article  PubMed  CAS  Google Scholar 

  5. Konwalinka, G.; Geissler, D.; Peschel, C., et al. Human erythropoiesis in vitro and the source of burst-promoting activity in a serum-free system. Exp. Hematol. 14:899–903; 1986.

    PubMed  CAS  Google Scholar 

  6. Migliaccio, G.; Migliaccio, A. R.; Adamson, J. W. The biology of hematopoietic growth factors: studies in vitro under serum-deprived conditions. Exp. Hematol. 18:1049–1055; 1990.

    PubMed  CAS  Google Scholar 

  7. Novogrodsky, A.; Katchalski, E. Induction of lymphocyte transformation by sequential treatment with neuraminidase and galactose oxidase. Proc. Natl. Acad. Sci. USA 70:1824–1827; 1973.

    Article  PubMed  CAS  Google Scholar 

  8. Sonoda, Y.; Yang, Y-C.; Wong, G. G., et al. Analysis in serum-free culture of the targets of recombinant human hemopoietic growth factors: interleukin 3 and granulocyte/macrophage-colony-stimulating factor are specific for early developmental stages. Proc. Natl. Acad. Sci. USA 85:4360–4364; 1988.

    Article  PubMed  CAS  Google Scholar 

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  1. Jiangxi Institute of Medical Sciences, 97 Beijing Road East, 330029, Nanchang, Jiangxi Province, People’s Republic of China

    Yu-cheng Dai, Zheng Fang & Xiao-quang Lu

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  1. Yu-cheng Dai
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  2. Zheng Fang
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  3. Xiao-quang Lu
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Dai, Yc., Fang, Z. & Lu, Xq. Clonogenic assay of leukemic cells in serum-free culture. In Vitro Cell Dev Biol - Animal 28, 685–686 (1992). https://doi.org/10.1007/BF02631048

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