Summary
Explants from neural tube and spinal cord of chick embryos at developmental stages 8 through 36 were cultured on collagen-coated cover glasses for 21 days. The cultures of neural tube at stages 10 to 14 contained many neuronal precursor cells which gave rise to nature neurons. This was verified by cumulative labeling of cultures with tritiated thymidine. Explants from spinal cords of stages 26 and 27 contained fewer precursor cells, and at stage 36, only 7% of mature neurons were labeled. Regardless of the stage of development at which explants were made (stages 8 through 36), all cultures had a similar appearance after 21 days, indicating that cells from explants taken from earlier developmental stages (before neurons were formed) “caught up” with the explants from later developmental stages, which already had formed neurons at the time of explantation.
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Portions of this work have been presented at scientific meetings (1, 2)
This work was supported by Grant No. MA 4235 from the Medical Research Council of Canada to Dr. S. Fedoroff; and during its progress, K. R. S. Fisher held a Medical Research Council Postdoctoral Fellowship
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Fisher, K.R.S., Fedoroff, S. The development of chick spinal cord in tissue culture. In Vitro 13, 569–579 (1977). https://doi.org/10.1007/BF02627853
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DOI: https://doi.org/10.1007/BF02627853