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Markers for human placental trophoblasts in two-dimensional gel electrophoresis

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Summary

Primary cultures of trophoblasts established from human term placentae showed high viability and reproducibility. Two-dimensional gel patterns obtained by metabolically labeling the trophoblasts with [35S]-methionine demonstrated that their pattern of gene expression was stable during the 6-d period investigated. Gel analysis demonstrated the keratins 7, 8, 14, 17, 18, and 19. Analysis of the gel pattern confirmed the presence of a small proportion of contaminating fibroblasts and lymphocytes. The gel patterns were compared with that of skin fibroblasts, peripheral lymphocytes, and epithelial cells to identify a group of proteins that are enriched in the trophoblasts and thus may be used as marker for these cells.

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Author notes

  1. Zheng Jie

    Present address: Department of Pathology, Nanjing Railway Medical College, Nanjing, Peoples Republic of China

Authors and Affiliations

  1. Department of Virus and Cancer, The Danish Cancer Society, Gustav Wieds Vej 10, DK-8000, Aarhus C, Denmark

    Zheng Jie, Henrik Hager & Peter Ebbesen

  2. Institute of Medical Microbiology and Institute of Human Genetics, University of Aarhus, DK-8000, Aarhus C, Denmark

    Stephen J. Fey & Peter Mose Larsen

  3. Institute of Medical Microbiology, University of Aarhus, DK-8000, Aarhus C, Denmark

    Per Høllsberg

Authors
  1. Zheng Jie
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  2. Stephen J. Fey
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  3. Henrik Hager
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  6. Peter Mose Larsen
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Additional information

This work was supported by the Danish Cancer Society, the Lundbeck Foundation, the Danish Medical Research Council, “Pedersholmlegatet,” and by “Anna og Jakob Jakobsens Legat.”

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Jie, Z., Fey, S.J., Hager, H. et al. Markers for human placental trophoblasts in two-dimensional gel electrophoresis. In Vitro Cell Dev Biol 26, 937–943 (1990). https://doi.org/10.1007/BF02624466

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