Summary
Two human osteosarcoma cell lines (TE-85, LM-1) were adapted to grow in serum-free hydrocortisone supplemented medium. Human osteosarcoma associated antigens were detected both on the cell membrane as well as in the concentrated culture medium. TE-85 cells produced bone specific alkaline phosphatase and LM-1 cells produced both bone specific and placental-like alkaline phosphatases when cultured in this medium. Successful proliferation of human osteosarcoma cell lines in serum-free medium is valuable in human osteosarcoma research.
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Research supported in part by U.S. Public Health Service Grant CA-29377 awarded by the National Cancer Institute, Bethesda, MD.
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Tsang, K.Y., Fudenberg, H.H., Sun, D.C. et al. Cultivation of human osteosarcoma cell lines in serum-free hormone supplemented medium. In Vitro 19, 515–521 (1983). https://doi.org/10.1007/BF02619599
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DOI: https://doi.org/10.1007/BF02619599