Summary
An organ explant culture system has been developed for long term maintenance of adult pancreatic tissue from the Syrian golden hamster. Gastric and duodenal lobe explants of up to 0.5 cm2 size were placed in tissue culture dishes (60 mm2) on Gelfoam sponge rafts to which was added 5 ml of CMRL medium 1066 supplemented with heat inactivated newborn bovine serum,l-glutamine hydrocortisone, insulin, and antibiotics. Dishes were placed in a controlled atmosphere chamber, which was gassed with 45% O2 50% N2, and 5% CO2 and incubated at 36.5°C. Viability of the tissues was determined by light and electron microscopy as well as by [3]thymidine incorporation. Explants were viable for up to 70 d. Zymogen granule-containing cells characteristic of acinar cells and mucuscontaining cells characteristic of ductal cells were present throughout this period. However, endocrine cells were only present for the 1st wk in culture.
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References
Silverberg, E. Cancer statistics 1982. Ca 32 (1): 3–30; 1982.
Wynder, E. An epidemiological evaluation of the causes of the pancreas. Cancer Res. 35: 2228–2233; 1975.
Lin, R. S.; Kesseler, I. I. A multifactoral method for pancreatic cover in man. JAMA 235; 147–152; 1981.
MacMahon, B.; Yen, S.; Trichopoulos D.; Warren K.; Nardi, G. Coffee and cancer of the pancreas. New Engl. J. Med. 304; 630–633; 1981.
Mack, J. M. Pancreas, Schottenfeld, D.; Fraumeni, J. F. eds. Cancer epidemiology and prevention. Philadelphia, PA: Saunders 1982: 638–667.
Pour, P.; Kruger, F.; Althoff, J.; Cardesa, A.; Mohr, U. Cancer of the pancreas induced in the syrian golden hamster. Am. J. Pathol. 76: 349–358; 1974.
Reddy, J. K.; Scarpelli, D. G.; Rao, M. S. Experimental pancreatic carcinogenesis. Adv. Med. Oncol. Res. Ed. 9: 99–109; 1979.
Jones, R. T.; Barrett, L. A.; van Haaften, C.; Harris, C. C.; Trump, B. F., Carcinogenesis of the pancreas. I. Long-term explant culture of the human and bovine pancreatic duct. J. Natl. Cancer Inst. 58; 557–565; 1977.
Anderson, A.; Borg, H.; Groth, G.; Gunvarsson, G.; Hellerstroni, C.; Lundgren, G.; Westman, J.; Ostman, J.; Survival of isolated human islets of Langerhans maintained in tissue culture. J. Clin. Invest. 57: 1295–1301; 1976.
Oliver, G. Isolation and maintenance of differentiated exocrine glands in vitro. In Vitro 16: 297–305; 1980.
Malik, L.; Tompa, A.; Kuszynsici, C.; Pour, P.; Langenbach, R. Maintenance of adult hamster pancreatic cells on fibroblastic cells. In Vitro 17: 947–955; 1981.
McDowell, E. M.; Trump, B. F. Histologic fixatives suitable for diagnostic light and electron microscopy. Arch. Pathol. Lab. Med. 100: 405–414; 1976.
Adams, C. W. A. Ap-dimethyl aminobenz-aldehyde nitrite method for the histochemical demonstration of tryptophane and related compounds. J. Clin. Pathol. 10: 56–63; 1957.
Boren, H.; Wright, G.; Harris, C. C.; Quantitative light microscopy. Autoradiography. Prescott, D. ed. Methods in cell biology, Vol. 8 New York: Academic Press, Inc.: 1974; 277–288.
Carrel, A.; Lindberg, C. The culture of organs. New York: Paul B. Hoeber, Inc.; 1938: 151.
McEvoy, R. C.; Hegre, O. D.; Lazarow, A. Fetal rat pancreas in organ islet cell components. Differentiation 6: 17–26: 1976.
Parsa, I.; Marsh, W. H. Long-term organ culture of embryonic rat pancreas in a chemically defined medium. Am. J. Pathol. 82: 119–128; 1976.
Hirata, K.; Oku, T.; Freeman, A. E.. Duct, exocrine, and endocrine components of cultured fetal mouse pancreas. In Vitro 18; 789–799; 1982.
Jones, R. T.; Trump, B. F. Cellular and subcellular effects of ischemia on the pancreatic acinar cell. In vitro studies of rat tissue. Virchows Arch. (Cell Pathol) 19: 325–336; 1975.
Jones, R. T.; Trump, B. F.; Stoner, G. D. Culture of human pancreatic ducts. Methods Cell Biol. 21B: 429–436; 1980.
De Duve, C. The lysosome in retrospect, Dingle, J. T.; Fell, H. B. eds. Lysosomes in biology and pathology, Vol. 1 Amsterdam North Holland: 1969; 3–40.
Nevalainen, T. J. Effects of pilocarpine stimulation on rat pancreatic acinar cells. An electron microscopic study with morphometric analysis. Acta Pathol. Microbiol. Scand. [A] 210: 9–43; 1970.
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This work was supported in part by National Cancer Institute Grant CA-19197-06 through the National Pancreatic Cancer Project and is UMP contribution No. 950.
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Resau, J.H., Hudson, E.A. & Jones, R.T. Organ explant culture of adult Syrian golden hamster pancreas. In Vitro 19, 315–325 (1983). https://doi.org/10.1007/BF02619510
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DOI: https://doi.org/10.1007/BF02619510