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Maintenance of adult hamster pancreas cells on fibroblastic cells

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Summary

The maintenance of primary cultures of adult hamster pancreatic cells on layers of irradiated C3H/10T1/2 cells was studied. Various types of pancreatic cells, acinar, islet and ductular cells could be identified in the cultures by light and electron microscopy. Morphologically the various pancreatic cells retained many differentiated characteristics of their respective in vivo cell types. Insulin production was maintained at near Day 1 levels for the 16 d in culture for which it was measured. Colonies of epithelial cells continued to grow during a 20 d culture period. It is believed that this procedure for maintaining functional and growing pancreas cells in culture may be a useful in vitro model for studying the initiation of pancreatic carcinogenesis.

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Authors and Affiliations

  1. Eppley Institute for Research in Cancer, University of Nebraska Medical Center, 42nd and Dewey Avenue, 68105, Omaha, Nebraska

    Linda E. Malick, Anna Tompa, Charles Kuszynski, Parviz Pour & Robert Langenbach

Authors
  1. Linda E. Malick
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  2. Anna Tompa
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  3. Charles Kuszynski
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  4. Parviz Pour
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  5. Robert Langenbach
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Additional information

Supported by Grant R01 CA 20022 and Contract N01 CP33278 from the National Cancer Institute, National Institutes of Health, Bethesda, Maryland.

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Malick, L.E., Tompa, A., Kuszynski, C. et al. Maintenance of adult hamster pancreas cells on fibroblastic cells. In Vitro 17, 947–955 (1981). https://doi.org/10.1007/BF02618419

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  • DOI: https://doi.org/10.1007/BF02618419

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