Summary
During serial subcultures 50 μg per ml gentamicin and penicillin (100 U per ml)-streptomycin (100 μg per ml) depressed cell growth significantly 2 weeks after the addition of the antibiotics; gentamicin, but not penicillin-streptomycin, stimulated cell growth before it became inhibitory. Removal of the antibiotics resulted in the cell yield returning to normal. The results show that these antibiotics can be harmful to cells even at concentrations thought to be safe.
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References
Rudin, A., A. Healey, C. A. Phillips, D. W. Gump, and B. R. Frosyth. 1970. Antibacterial activity of gentamicin sulfate in tissue culture. Appl. Microbiol. 20: 989–990.
Braun, P., J. O. Klein, and E. H. Kass. 1970. Susceptibility of genital mycoplasmas to antimicrobial agents. Appl. Microbiol. 19: 62–70.
Perlman, D., S. B. Rahman, and J. B. Semar. 1967. Antibiotic control of mycoplasma in tissue culture. Appl. Microbiol. 15: 82–85.
Perlman, D. 1976. Some guidelines for selection of antibiotics for elimination of unwanted microbial contaminants in tissue cultures. Procedure 15143. TCA Manual 2: 383–386.
Byarugaba, W., H. W. Rüdiger, T. Koske-Westphal, W. Wöhler, and E. Passarge. 1975. Toxicity of antibiotics on cultured human skin fibroblasts. Humangenetik 28: 263–267.
Schafer, T. W., A. Pascale, G. Shimonaski, and P. E. Came. 1972. Evaluation of gentamicin for use in virology and tissue culture. Appl. Microbiol. 23: 565–570.
Goetz, I. E. 1975. Growth of human skin fibroblasts from punch biopsies. Procedure 41523. TCA Manual 1: 13–15.
Fischer, A. B. 1975. Gentamicin as a bactericidal antibiotic in tissue culture. Med. Microbiol. Immunol. 161: 23–39.
Litwin, J. 1970. The effect of commercial and pure gentamicin on the growth of human diploid lung fibroblasts. Acta Pathol. Microbiol. Scand. Sec. B 78: 273–276.
Tulkens, P., and A. Trouet. 1974. Uptake and intracellular localization of kanamycin and gentamicin in the lysosomes of cultured fibroblasts. Soc. Belge de Biochimie, Reunion de Louvain-la-Neuve, pp. 1018–1019.
Tulkens, P., and A. Trouet. 1972. Uptake and intracellular localization of streptomycin in the lysosomes of cultured fibroblasts. Soc. Belge de Biochimie, Reunion de Strasbourg, pp. 623–624.
Coriell, L. L. 1973. Methods of prevention of bacterial, fungal, and other contaminations. In: Jorgen Fogh (Ed.),Contamination in Tissue Culture. Academic Press, London, pp. 29–49.
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Goetz, I.E., Moklebust, R. & Warren, C.J. Effects of some antibiotics on the growth of human diploid skin fibroblasts in cell culture. In Vitro 15, 114–119 (1979). https://doi.org/10.1007/BF02618107
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DOI: https://doi.org/10.1007/BF02618107