Abstract
The number of mouseTcra-V gene segments varies from one individual to another and is estimated to be about 100. Southern blot analysis revealed that most of theTcra-V are organized in clusters composed of copies ofTcra-V belonging to different subfamilies. We analyzed in detail aTcra-V subfamily and looked for newTcra-V in order to improve the knowledge of the mouseTcra locus organization. A series of genomic clones derived from the B10.A mouse strain enclosing these clusters was used to determined the structure of all theTcra-V2. We were able to identify tenTcra-V2. This study showed that theTcra-V2 can be organized into three structural subgroups. The distribution of the genes along theTcra locus, plus their structural organization, indicates that successive duplications occurred during the processes of expansion and contraction of theTcra-V gene subfamilies. SeveralTcra-V2 are also identical, indicating recent duplications. The most divergentTcra-V2 differ by 7.4% nucleotides, leading to 5.2% differences in amino acid contents.
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The nucleotide sequence data reported in this paper have been submitted to the Genome Sequence Data Base (GSDB) and have been assigned the accession numbers L77148 (Tcra-V2.1), L77149 (Tcra-V2.2), L77150 (Tcra-V2.3), L77151 (Tcra-V2.4), L77152 (Tcra-V2.5), L77153 (Tcra-V2.6), and L77154 (Tcra-V2.7)
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Gahéry-Ségard, H., Jouvin-Marche, E., Six, A. et al. Germline genomic structure of the B10.A mouseTcra-V2 gene subfamily. Immunogenetics 44, 298–305 (1996). https://doi.org/10.1007/BF02602560
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DOI: https://doi.org/10.1007/BF02602560