Summary
Metabolism of polyamines (spermidine and spermine) is known to be strictly related to the growth processes of eukaryotic cells. Since cell replication processes appear altered in insulin-dependent diabetes mellitus (IDDM), especially when associated with its microvascular complications, the aim of this study was measuring serum spermidine oxidase activity (SOA), a key enzyme in the metabolic pathway of polyamines, in 47 patients with IDDM as compared with 63 healthy control subjects matched for age and sex. Mean SOA levels±SD were significantly lower in IDDM patients (177.4±57.2 µkat/l) than in controls (247.6±68.1 µkat/l; p<0.001), being SOA inversely related with daily insulin dose. SOA was moreover significantly higher (but similar to controls) in the group with increased urinary albumin excretion rate (AER persistently >20 µg/min); (n=17; 213.1±62.6 µkat/l) in comparison with normoalbuminuric subjects (n=30; 156.6±43.5 µkat/l; F=21.78; p=0.0001). SOA was correlated with AER (r=0.45; p=0.001), independently of age, duration of disease, serum creatinine, body weight, blood pressure and metabolic control, as shown by a multiple regression analysis model (p=0.003). Presence of background retinopathy was not associated with modified levels of SOA, which was conversely higher, although not significantly, in the patients with proliferative retinal lesions. In conclusion serum SOA is deeply altered in IDDM patients, being markedly reduced in the whole group of patients and conversely independently increased up to the mean values of controls in presence of increased AER or advanced retinopathy.
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References
Bending J. J., Lobo-Yeo A., Vergani D., Viberti G. C.: Proteinuria and activated T-lymphocytes in diabetic nephropathy — Diabetes37, 507–511, 1988.
Byrd J., Jacobs D. M., Amoss M. S.: Synthetic polyamines added to cultures containing bovine sera, reversibly inhibitin vitro parameters of immunity — Nature (Lond.)267, 621–623, 1977.
Conover C. A., Rozovski S. J., Belur E. L., Aoki T. T., Ruderman N. B.: Ornithine decarboxylase activity in insulin-deficient states — Biochem. J.192, 725–732, 1980.
Flayeh K. A.: Spermidine oxidase activity of normal and schizophrenic subjects — Clin. Chem.34, 401–403, 1988.
Gahl A., Vale A. M., Pitot H. C.: Spermidine oxidase in human pregnancy serum — Biochem. J.201, 161–166, 1982.
Gaugas M., Curzen P.: Polyamine interaction with pregnancy serum in suppression of lymphocyte transformation — Lanceti, 18–20, 1978.
Groop L. C., Teir H., Koskimies S., Groop P. H., Matikainen E., Verkkala E., Scheinin T., Kontianen S., Teppo A. M., Tolppanen E. M., Tallgren L. G.: Risk factors and markers associated with proliferative retinopathy in patients with insulin-dependent diabetes — Diabetes35, 1397–1403, 1986.
Illei G., Morgan D. M. L.: Polyamine oxidase activity in human pregnancy serum — Brit. J. Obstet. Gynaecol.86, 878–881, 1979.
Janne J., Poso H., Raina A.: Polyamines in rapid growth and cancer — Biochim. biophys. Acta (Amst.)473, 241–293, 1970.
Mondovì B., Riccio P.: Animal intracellular amine oxidases. In:Mondoví B. (Ed.): Structure and Functions of Amine Oxidases. CRC Press, Boca Raton/FL, 1985; pp. 63–76.
Mondovì B., Riccio P., Agostinelli E.: The biological functions of amine oxidases and their reaction products: an overview. In:Zappia V., Pegg A. E. (Eds): Progress in Polyamine Research. Plenum Press, New York, 1988; pp. 147–171.
National Diabetes Data Group: Classification and diagnosis of diabetes mellitus and other categories of glucose intolerance — Diabetes28, 1039–1057, 1979.
Petty R. G., Pearson J. D., Morgan D. M. L., Mahler R. F.: Stimulation of endothelial cell growth by sera from diabetic patients with retinopathy — Lanceti, 208–211, 1988.
SAS Institute Inc.: SAS/STAT Guide for Personal Computers. 6th Ed. SAS Institute Inc., Cary/NC, 1987.
Scheinen T., Groop L., Teir H., Koskimies S., Kontianen S.: HLA-types and insuline responses in insulin dependent diabetics with and without proliferative retinopathy — J. clin. Lab. Immunol.18, 17–20, 1985.
Trevisan R., Li K. L., Walker J. D., Viberti G. C.: Overactivity of Na+/H+ antiport and enhanced cell growth in fibroblasts of type 1 (insulin-dependent) diabetic patients with nephropathy — Diabetologia32, 549A, 1989.
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Seghieri, G., Gironi, A., Niccolai, M. et al. Serum spermidine oxidase activity in patients with insulin-dependent diabetes mellitus and microvascular complications. Acta diabet. lat 27, 303–308 (1990). https://doi.org/10.1007/BF02580934
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DOI: https://doi.org/10.1007/BF02580934