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Changes in concentrations of methylglyoxal,d-lactate and glyoxalase activities in liver and plasma of rats fed a 3′-methyl-4-dimethylaminoazobenzene-rich diet

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Research in Experimental Medicine

Abstract

Donryu male albino rats were fed a diet containing 0.064% 3′-methyl-4-dimethylaminoazobenzene (MDAB) for 21 weeks. During the ensuing rat liver carcinogenesis, changes in the concentrations of methylglyoxal,d-lactate and glutathione as well as activities of glyoxalase I and II in liver and plasma were examined. After the start of the diet, hepatic contents of methylglyoxal andd-lactate increased to about 7 and 3 times that of the control, respectively. However, after 21 weeks thed-lactate content decreased from the elevated level, but remained at a higher level of 1.4 times the control. The hepatic glyoxalase I activity increased 1.2 to 1.7 times over the control during carcinogenesis, while glyoxalase II activity increased 160% during the precancerous state and decreased to 55% of control at 21 weeks. The hepatic level of reduced glutathione (GSH) increased and peaked after 4 weeks of the MDAB diet and decreased thereafter to 57% of the control level after 21 weeks. Both pyruvate andl-lactate levels increased in the liver and plasma of MDAB-fed rats when rats had obvious symptoms of hepatoma.

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References

  1. Ayoub F, Zaman M, Thornalley P, Masters J (1993) Glyoxalase activities in human tumour cell linesin vitro. Anticancer Res 13:151–156

    PubMed  CAS  Google Scholar 

  2. Beisenherz G, Boltze HJ, Bücher Th, Czok R, Garbade KH, Meyer-Arendt E, Pfleiderer G (1953) Biuret Methode zur Proteinbestimmung. Z Naturforsch 8b:555–577

    CAS  Google Scholar 

  3. Dakin HD, Dudley HW (1913) On glyoxalase. J Biol Chem 14:423–431

    CAS  Google Scholar 

  4. Együd LG, Szent-Györgyi A (1966) On the regulation of cell division. Proc Natl Acad Sci USA 56:203–207

    Article  PubMed  Google Scholar 

  5. Együd LG, Szent-Györgyi A (1966) Cell division, SH, ketoaldehydes, and cancer. Proc Natl Acad Sci USA 55:388–393

    Article  PubMed  Google Scholar 

  6. Giese JE, Miller JA, Baumann CA (1945) The carcinogenicity ofm′-methyl-p-dimethylaminoazobenzene and ofp-monomethyl-aminoazobenzene. Cancer Res 5:337–340

    CAS  Google Scholar 

  7. Jerzykowski T, Matuszewski W, Otrzonsek N, Winter R (1970) Antineoplastic action of methylglyoxal. Neoplasma 17:25–35

    PubMed  CAS  Google Scholar 

  8. Jerzykowski T, Matuszewski W, Winter R (1974) Glyoxalase system enzyme activity in the liver of rats fed dimethylaminoazobenzene. Neoplasma 21:679–683

    PubMed  CAS  Google Scholar 

  9. Jerzykowski T, Winter R, Matuszewski W, Szczurek Z (1975) Glyoxalase II activity in tumours Experientia 31:32–33

    Article  PubMed  CAS  Google Scholar 

  10. Kasai H, Kumeno K, Yamaizumi Z, Nishimura S, Nagao M, Fujita Y, Sugimura T, Nukata K, Kosuge T (1982) Mutagenicity of methylglyoxal in coffee. Gann 73:681–683

    PubMed  CAS  Google Scholar 

  11. Kawase M, Kondoh C, Matsumoto S, Teshigawara M, Chisaka Y, Higashiura M, Nakata K, Ohmori S (1995) Contents ofd-lactate and its related metabolites as well as enzyme activities in the liver, muscle and blood plasma of aging rats. Mech Ageing Dev 84:55–63

    Article  PubMed  CAS  Google Scholar 

  12. Kondoh Y, Kawase M, Kawakami Y, Ohmori S (1992) Concentrations ofd-lactate and its related metabolic intermediates in liver, blood, and muscle of diabetic and starved rats. Res Exp Med 192:407–414

    CAS  Google Scholar 

  13. Kondoh Y, Kawase M, Ohmori S (1992)d-Lactate concentrations in blood, urine and sweat before and after exercise. Eur J Appl Physiol 65:88–93

    Article  CAS  Google Scholar 

  14. Kondoh Y, Kawase M, Hirata M, Ohmori S (1994) Carbon sources ford-lactate formation in rat liver. J Biochem 115:590–595

    PubMed  CAS  Google Scholar 

  15. Krymkiewicz N, Diéguez E, Rekarte UD, Zwaig N (1971) Properties and mode of action of a bactericidal compound (=methylglyoxal) produced by a mutant ofEscherichia coli. J Bacteriol 108:1338–1347

    PubMed  CAS  Google Scholar 

  16. Lo TWC, Westwood ME, McLellan AC, Selwood T, Thornalley PJ (1994) Binding and modification of proteins by methylglyoxal under physiological conditions. J Biol Chem 269:32299–32305

    PubMed  CAS  Google Scholar 

  17. Matsumoto S, Teshigawara M, Tsuboi S, Ohmori S (1996) Determination of glutathione and glutathione disulfide in biological samples using acrylonitrile as a thiol-blocking reagent. Anal Sci 12:91–95

    CAS  Google Scholar 

  18. Neish WJP, Rylett A (1963) Effect of a-tocopheryl acetate on liver glutathione of male rats injected with 3′-methyl-4-dimethylaminoazobenzene. Biochem Pharmacol 12:1147–1150

    Article  PubMed  CAS  Google Scholar 

  19. Neish WJP, Rylett A (1963) Azo dyes and rat liver glutathione. Biochem Pharmacol 12:893–903

    Article  PubMed  CAS  Google Scholar 

  20. Neish WJP, Davies HM, Reeve PM (1964) Carcinogenic azo dyes, dye-binding and liver glutathione. Biochem Pharmacol 13:1291–1303

    Article  PubMed  CAS  Google Scholar 

  21. Neuberg C (1913) Weitere Untersuchungen über die biochemische Umwandlung von Methylglyoxal in Milchsäure nebst Bemerkungen über die Entstehung der verschiedenen Milchsäuren in der Natur. Biochem Z 51:484–508

    Google Scholar 

  22. Ohmori S, Kawase M, Mori M, Hirota T (1987) Simple and sensitive determination of methylglyoxal in biological samples by gas chromatography with electron-capture detection. J Chromatogr 415:221–229

    Article  PubMed  CAS  Google Scholar 

  23. Ohmori S, Mori M, Kawase M, Tsuboi S (1987) Determination of methylglyoxal as 2-methylquinoxaline by high-performance liquid chromatography and its application to biological samples. J Chromatogr 414:149–155

    Article  PubMed  CAS  Google Scholar 

  24. Ohmori S, Iwamoto T (1988) Sensitive determination ofd-lactic acid in biological samples by high-performance liquid chromatography. J Chromatogr 431:239–247

    Article  PubMed  CAS  Google Scholar 

  25. Ohmori S, Mori M, Shiraha K, Kawase M (1989) Biosynthesis and degradation of methylglyoxal in animals. Biol Res 290:397–412

    CAS  Google Scholar 

  26. Ohmori S, Nose Y, Ogawa H, Tsuyama K, Hirota T (1991) Fluorimetric and high-performance liquid chromatographic determination ofd-lactate in biological samples. J Chromatogr 566:1–8

    Article  PubMed  CAS  Google Scholar 

  27. Racker E (1955) Glyoxalases. Methods Enzymol 454–460

  28. Scaife JF (1969) Mitotic inhibition induced in human kidney cells by methylglyoxal and kethoxal. Experientia 25:178–179

    Article  PubMed  CAS  Google Scholar 

  29. Takahashi K (1977) The reactions of phenylglyoxal and related reagents with amino acids. J Biochem 81:395–402

    PubMed  CAS  Google Scholar 

  30. Teshigawara M, Matsumoto S, Tsuboi S, Ohmori S (1995) Changes in levels of glutathione and related compounds and activities of glutathione-related enzymes during rat liver regeneration Res Exp Med 195:55–60

    CAS  Google Scholar 

  31. Thornalley PJ (1993) The glyoxalase system in health and disease. Mol Aspects Med 14:288–371

    Google Scholar 

  32. Valentine WN, Tanaka KR (1966) Pyruvate kinase: clinical aspects. Methods Enzymol 9:468–473

    Article  CAS  Google Scholar 

  33. Weber AL (1982) Formation of the thioester,N-acetyl,S-lactoylcysteine, by reaction ofN-acetylcysteine with pyruvaldehyde in aqueous solution. J Mol Evol 18:354–359

    Article  PubMed  CAS  Google Scholar 

  34. Winter R, Piskorska D, Jerzykowski T (1978) Further studies on liver glyoxalase I and glyoxalase II. Activity in mice bearing sarcoma 180 and L1210 leukemia. Neoplasma 25:465–470

    PubMed  CAS  Google Scholar 

  35. Wlodek L, Wrobel M (1992) Effect of aldehydes on free sulfhydryl groups and glycolysis in extracts of Ehrlich ascites tumor cells and mouse liver. Neoplasma 39:223–228

    PubMed  CAS  Google Scholar 

  36. Yamaguchi T (1982) Mutagenicity of trioses and methylglyoxal onSalmonella typhimurium. Agric Biol Chem 46:849–851

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka-1, 700, Okayama, Japan

    M. Kawase, M. Tada, S. Akagi & S. Ohmori

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  1. M. Kawase
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  2. M. Tada
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  3. S. Akagi
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  4. S. Ohmori
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Kawase, M., Tada, M., Akagi, S. et al. Changes in concentrations of methylglyoxal,d-lactate and glyoxalase activities in liver and plasma of rats fed a 3′-methyl-4-dimethylaminoazobenzene-rich diet. Res. Exp. Med. 196, 251–259 (1996). https://doi.org/10.1007/BF02576849

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  • DOI: https://doi.org/10.1007/BF02576849

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