Skip to main content
SpringerLink
Log in

Rat model of mild stenosis of the common bile duct

  • Original Papers
  • Published:
Research in Experimental Medicine

Abstract

Several techniques for developing incomplete obstruction of the common bile duct have been described but none of them properly represents a compression or constriction of the bile duct. In this study, a mild stenosis of the common bile duct was achieved in the rat by means of a double ligature including a cannula that could be easily slipped out of the ligatures. Sham-operated rats were used as controls. The studies, performed 7–10 days postoperatively, indicated that in ligated rats a duct constriction was produced, made evident by an increase of the biliary pressure, an upstream dilatation of the bile duct, an increase of the liver volume constituted by portal tracts, and ductular proliferation. Serum parameters were practically similar in ligated and control rats, except for a slight increase in serum bilirubin. Following intravenous injection of sodium taurocholate there were rapid increases of bile flow and bile salt output in both groups, but choleresis induced by sodium taurocholate was higher in ligated rats than in controls. The clearances of [14C]erythritol and [14C]sucrose suggested that ductular water contributing to bile flow and changes in biliary permeability were not involved in ligated rats. The limited repercussion of humoral effects and hepatic behaviour seen in ligated rats despite the morphological alterations induced make the mild stenosis of the bile duct a good model for the study of early stages of compression or constriction of the biliary tract.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Accatino L, Gavilan P, Contreras A, Quintana C (1984) Effects of increased biliary resistance on bile secretion in the rat. J Lab Clin Med 104:51–59.

    PubMed  CAS  Google Scholar 

  2. Alpini G, Lenzi R, Sarkozi L, Tavoloni N (1988) Biliary physiology in rats with bile ductular cell hyperplasia. Evidence for a secretory function of proliferated bile ductules. J Clin Invest 81:569–578

    Article  PubMed  CAS  Google Scholar 

  3. Angelini G, Sgarbi D, Castagnini P, Cavallini G, Bovo P (1994) Common bile duct involvement in chronic pancreatitis. Ital J Gastroenterol 26:79–82

    PubMed  CAS  Google Scholar 

  4. Anwer MS, Hegner D (1982) Importance of solvent drag and diffusion in bile acid-dependent bile formation: ion substitution studies in isolated perfused rat liver. Hepatology 2:580–586

    PubMed  CAS  Google Scholar 

  5. Batta AK, Shefer S, Salen G (1981) Thin-layer chromatographic separation of conjugates of ursodeoxycholic acid from those of litho-, chenodeoxy-, deoxy-, and cholic acids. J Lipid Res 22:712–714

    PubMed  CAS  Google Scholar 

  6. Berthelot P, Erlinger S, Dhumeaux D, Preaux AM (1970) Mechanism of phenobarbital induced hypercholeresis in the rat. Am J Physiol 219:809–813

    PubMed  CAS  Google Scholar 

  7. Besançon F (1960) Obstruction biliaire graduée expérimentale. I Le débit et la pression. C Seances Soc Biol Paris 154:564–566

    Google Scholar 

  8. Burt AD, MacSween RNM (1993) Bile duct proliferation. Its true significance? Histopathology 23:599–602

    PubMed  CAS  Google Scholar 

  9. Cooper AD, Jones AL, Koldinger RE, Ockner RK (1974) Selective biliary obstruction. A model for the study of lipid metabolism in cholestasis. Gastroenterology 66:574–585

    PubMed  CAS  Google Scholar 

  10. Dubin M, Maurice M, Feldman MG, Erlinger S (1980) Influence of colchicine and phalloidin on bile secretion and hepatic ultrastructure in the rat. Possible interaction between microtubules and microfilaments. Gastroenterology 79:646–654

    PubMed  CAS  Google Scholar 

  11. Frezza EE, Gerunda GE, Plebani A, Galligioni A, Giacomini A, Neri D, Faccioli AM, et al. (1993) Effect of ursodeoxycholic acid administration on bile duct proliferation and cholestasis in bile duct ligated rat. Dig Dis Sci 38:1291–1296

    Article  PubMed  CAS  Google Scholar 

  12. Gaebler OH (1945) Determination of bromsulphthalein in normal, turbid, hemolyzed or icteric serums. Am J Clin Pathol 15:452–455

    CAS  Google Scholar 

  13. Graf J (1983) Canalicular bile salt-independent bile formation: concepts and clues from electrolyte transport in rat liver. Am J Physiol 244:G233–246

    PubMed  CAS  Google Scholar 

  14. Hatoff DE, Hardison WGM (1981) Bile acids modify alkaline phosphatase induction and bile secretion pressure after bile duct obstruction in the rat. Gastroenterology 80:666–672

    PubMed  CAS  Google Scholar 

  15. Hornstein B, Stammler L, Bianchi L, Landmann L (1992) Ethinylestradiol increases volume and decreases sinusoidal membrane surface in the rat liver: a stereological analysis. Hepatology 217–223

  16. Jaeschke H, Krell H, Pfaff E (1987) Quantitative estimation of transcellular and paracellular pathways of biliary sucrose in isolated perfused rat liver. Biochem J 241:G35–40

    Google Scholar 

  17. Jendrassik J, Grof P (1938) Vereinfachte photometrische Methode zur Bestimmung des Blutbilirubins. Biochem Z 297:81–89

    CAS  Google Scholar 

  18. Klaassen CD (1971) Studies on the increased biliary flow produced by phenobarbital in rats. J Pharmacol Exp Ther 176:743–751

    PubMed  CAS  Google Scholar 

  19. Lesur G, Levy Ph, Flejou JF, Belghiti F, Fekete P, Bernades P (1993) Factors predictive of liver histopathological appearance in chronic alcoholic pancreatitis with common bile duct stenosis and increased serum alkaline phosphatase. Hepatology 18:1078–1081

    PubMed  CAS  Google Scholar 

  20. Marinelli RA, Roma MG, Pellegrino JM, Rodríguez Garay EA (1992) Taurolithocholate-induced inhibition of biliary lipid and protein excretion in the rat. Biochim Biophys Acta 1125:44–48

    PubMed  CAS  Google Scholar 

  21. Ostrow JD (1993) Metabolism of bile salts in cholestasis in humans. In: Tavoloni N, Berk PD (eds) Hepatic transport and bile secretion: physiology and pathophysiology, Raven Press, New York, pp 46:673–712

    Google Scholar 

  22. Reichen J Le M (1983) Taurocholate, but not taurodehydrocholate, increases biliary permeability to sucrose. Am J Physiol 245:G651–655

    PubMed  CAS  Google Scholar 

  23. Rodríguez-Garay EA, Spetale MR, Robledo HA, Morisoli LS, Rassero JA (1972) Estudios sobre la función hepática en ratas tratadas con rifampicina oral durante períodos prolongados. II. Farmaco 27:633–642

    Google Scholar 

  24. Roma MG, Carnovale C, Rodríguez-Garay EA (1992) Bile salt-associated electrolyte secretion. A study following a short-term biliary obstruction in the rat. Exp Toxicol Pathol 44:354–360

    PubMed  CAS  Google Scholar 

  25. Roma MG, Luque EA, Marinelli RA, Rodríguez Garay EA (1989) A simple method for the correction of biliary excretion curves distorted by the biliary dead space. Biochem Pharmacol 38:4021–4025

    Article  PubMed  CAS  Google Scholar 

  26. Sathirakul K Suzuki H, Yamada T, Hanano M, Sugiyama Y (1994) Multiple transport systems for organic anions across the bile canalicular membrane. J Pharmacol Exper Ther 268:65–73

    CAS  Google Scholar 

  27. Snedecor GW, Cochram WG (1962) Statistical methods, 6th edn. Iowa State University Press, Iowa City, pp 419–439

    Google Scholar 

  28. Strasberg SM, Ilson RG, Paloheimo JE (1983) Bile salt-associated electrolyte secretion and the effect of sodium taurocholate on bile flow. J Lab Clin Med 101:317–326

    PubMed  CAS  Google Scholar 

  29. Talalay P (1960) Enzymatic analysis of steroid hormones. Methods Biochem Anal, 8:119–143

    Article  PubMed  CAS  Google Scholar 

  30. Tavoloni N (1987) The intrahepatic biliary epithelium: an area of growing interest in hepatology. Semin Liver Dis 7:280–292

    Article  PubMed  CAS  Google Scholar 

  31. Van Thiel DH, Gavaler JS, Slone FL, Cobb ChF, Smith WI Jr, Bron KM Lester R (1980) Is feminization in alcoholic men due in part to portal hypertension: a rat model. Gastroenterology 78:81–91

    PubMed  Google Scholar 

  32. Weibel ER (1979) Practical methods for biological morphometry. (Stereological methods, vol I) Academic Press, London

    Google Scholar 

  33. Whelan FJ, Plaa GL (1963) The application of thin layer chromatography to sulfobromophthalein metabolism studies. Toxicol Appl Pharmacol 5:457–463

    Article  PubMed  CAS  Google Scholar 

  34. Zimmerman H, Hugi A, Reichen J (1994) The effect of increasing biliary stenosis on hepatic structure in the rat (abstract). Hepatology 19:144

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto de Fisiologia Experimental, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Universidad Nacional de Rosario, Suipacha 570, 2000, Rosario, Argentina

    Emilio A. Rodríguez-Garay, Rut M. Agüero, Gerardo Pisani, Raul A. Trbojevich, Abel Farroni & Ricardo A. Viglianco

Authors
  1. Emilio A. Rodríguez-Garay
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rut M. Agüero
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gerardo Pisani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Raul A. Trbojevich
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Abel Farroni
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ricardo A. Viglianco
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rodríguez-Garay, E.A., Agüero, R.M., Pisani, G. et al. Rat model of mild stenosis of the common bile duct. Res. Exp. Med. 196, 105–116 (1996). https://doi.org/10.1007/BF02576832

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02576832

Key words

Search

Navigation