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Patterns of meiotic double-strand breakage on native and artificial yeast chromosomes

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Abstract

The preferred positions for meiotic double-strand breakage were mapped onSaccharomyces cerevisiae chromosomes I and VI, and on a number of yeast artificial chromosomes carrying human DNA inserts. Each chromosome had strong and weak double-strand break (DSB) sites. On average one DSB-prone region was detected by pulsed-field gel electrophoresis per 25 kb of DNA, but each chromosome had a unique distribution of DSB sites. There were no preferred meiotic DSB sites near the telomeres. DSB-prone regions were associated with all of the known “hot spots” for meiotic recombination on chromosomes I, III and VI.

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Authors and Affiliations

  1. Department of Genetics, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel

    Shoshana Klein, Drora Zenvirth, Vardit Dror & Giora Simchen

  2. Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Graduate School of Biomedical Sciences, 07103, Newark, NJ, USA

    Arnold B. Barton & David B. Kaback

Authors
  1. Shoshana Klein
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  2. Drora Zenvirth
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  3. Vardit Dror
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  4. Arnold B. Barton
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  5. David B. Kaback
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  6. Giora Simchen
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Correspondence to Giora Simchen.

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Edited by: J.-L. Rossignol

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Klein, S., Zenvirth, D., Dror, V. et al. Patterns of meiotic double-strand breakage on native and artificial yeast chromosomes. Chromosoma 105, 276–284 (1996). https://doi.org/10.1007/BF02524645

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  • DOI: https://doi.org/10.1007/BF02524645

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