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HPLC Analysis of the flavonoids in pharmaceutical preparations from canadian goldenrod (Solidago canadensis)

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Summary

Solidago canadensis L., Canadian goldenrod (Asteraceae) has been used in European phytotheraphy for centuries as a component of urological and antiphlogistical remedies. High-performance liquid chromatography (HPLC) coupled with diode-array detection (DAD) and online mass spectrometry (MS) has been used for the separation and quantification of phenolics (chlorogenic acid, caffeic acid, kaempferol-3-O-α-L-rutinoside (nicotiflorin), quercetin-3-O-β-D-rutinoside (rutin), quercetin-3-O-β-D-galactoside (hyperoside), quercetin-3-O-β-D-glucoside (isoquercitrin), quercetin-3-O-β-D-rhamnoside (quercitrin), kaempferol-3-O-α-L-rhamnoside (afzelin) and quercetin from Solidaginis herba. Extracts have been obtained using different technologies. Three aqueous and three alcoholic extracts were studied separately. Reversedphase high-performance liquid chromatography separation of polyphenols on octadecyl sorbent Hypersil was performed, using acetonitrile: acetic acid 2.5 v/v % as eluent in gradient elution. Our results confirm previous reports concerning the presence of several flavonoids. Quantification of the main quercetin glycosides in pharmaceuticals is also reported.

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Authors and Affiliations

  1. Faculty of Pharmacy, Semmelweis University, Department of Pharmacognosy, Üllői út 26, 1085, Budapest, Hungary

    P. Apati, A. Balázs, T. Sz. Kristó, É. Szőke & Á. Kéry

  2. Institute of Chemistry, Hungarian Academy of Sciences, Chemical Research Center, Pusztaszeri út 59-67, 1025, Budapest, Hungary

    P. Apati & K. Szentmihályi

  3. Pharmaceutical Biology, Institute of Pharmacy, Friedrich-Schiller University, Semmelweis Str. 10, 07743, Jena, Germany

    D. Baumann & M. Hamburger

Authors
  1. P. Apati
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  2. K. Szentmihályi
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  3. A. Balázs
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  4. D. Baumann
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  5. M. Hamburger
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  6. T. Sz. Kristó
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  7. É. Szőke
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  8. Á. Kéry
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Apati, P., Szentmihályi, K., Balázs, A. et al. HPLC Analysis of the flavonoids in pharmaceutical preparations from canadian goldenrod (Solidago canadensis). Chromatographia 56, S65–S68 (2002). https://doi.org/10.1007/BF02494115

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  • DOI: https://doi.org/10.1007/BF02494115

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