Determination of hesperidin and naringin by micellar electrokinetic chromatography using a new recording mode

Summary

A micellar electrokinetic chromatographic(MEKC) method for determining hesperdin (HP) and Naringin (NG) in Chinese traditional medicines, Zhike and Zhishi, has been established. A new capillary-electrophoresis recording made using the electric flux domain depending on the current values was employed. The rate of electroosmotic flow was obtained through the abrual change of the electriccurrent, and the results demonstrated the proposed method were better than the traditional neutral marker method. The optimum buffer system was 20 mM borate −60 mM sodium dodecylsulfate (SDS) −5% (v/v) methanol (pH 9.5). The voltage was 14 kV and detection at 214 nm. The effects of borax and SDS concentration, pH value and organic madifies were studied systematically. The lower limits of detection (defined as a signalto-noise ratio of ca. 3) were approximately 2.55 mg L⁻¹ for HP and 1.46 mg L⁻¹ for NG. The relative standard deviation of the electric flux and peak area of the hesperidin and Naringin were 0.28, 2.2 and 0.27, 1.9%, respectively and the recoveries of the two compounds ronged between 95–104% for HP and 92–104% for NG.