Skip to main content
SpringerLink
Log in

A fluorimetric liquid chromatography for highly sensitive analysis of very long chain fatty acids as naphthoxyethyl derivatives

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

A simple and sensitive liquid chromatographic method is described for the simultaneous determination of biologically important very long chain fatty acids (docosanoic, tetracosanoic and hexacosanoic acids) as fluorogenic derivatives. The method is based on the derivatization of the fatty acids with 2-(2-naphtoxy)ethyl 2-(piperidino)ethanesulfonate (NOEPES) in toluene in the presence of potassium carbonate and 18-crown-6. Several parameters affecting the derivatization were studied, including reaction temperature, reaction time, reaction solvent, base catalyst and the amount of the reagent. The resulting derivatives were analyzed by HPLC with fluorimetric detection (λex=235 nm; λem=366 nm). The linear range for the determination of docosanoic, tetracosanoic and hexacosanoic acids was 0.028–1.4 μM with a detection limit of about 5.6 nM (S/N=3) (56 fmol per 10 μL injection). Application of the method to the analysis the non-esterified (free) very long chain fatty acids spiked in plasma proved feasible.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. H. W. Moser, A. B. Moser, K. K. Frayer, W. Chen, J. D. Schulman, B. P. O’Neill, Y. Kishimoto, Neurology,31, 1241 (1981).

    CAS  Google Scholar 

  2. O. Lazo, M. Contreras, M. Hashmi, W. Stanley, C. Irazu, I. Singh, Proc. Natl. Acad. Sci.85, 7647 (1988).

    Article  CAS  Google Scholar 

  3. N. A. Hall, G. W. Lynes, N. M. Hjelm, Clin. Chem.,34(6), 1041 (1988).

    CAS  Google Scholar 

  4. P. Aubourg, P. F. Bougneres, F. Rocchiccioli, J. Lipid Res.26, 263 (1985).

    CAS  Google Scholar 

  5. P. Vreken, A. E. M. van Lint, A. H. Bootsma, H. Overmars, R. J. A. Wanders, A. H. van Gennip, J. Chromatogr. B.713, 281 (1998).

    CAS  Google Scholar 

  6. F. Stellaard, H. J. ten Brink, R. M. Kok, L. Van den Heuvel, C. Jakobs, Clin. Chim. Acta192, 133 (1990).

    Article  CAS  Google Scholar 

  7. W. Onkenhout, P. F. H. van der Poel, M. P. M. van den Heuvel, J. Chromatogr.494, 31 (1989).

    CAS  Google Scholar 

  8. U. Caruso, B. Fowler, M. Erceg, C. Romano, J. Chromatogr.562, 147 (1991).

    CAS  Google Scholar 

  9. R. B. H. Schutgens, I. W. Bouman, A. A. Nijenhuis, R. J. A. Wanders, M. E. J. Frumau, Clin. Chem.39 (8), 1632 (1993).

    CAS  Google Scholar 

  10. H. Vallance, D. Applegarth, Clin. Biochem.27, 183 (1994).

    Article  CAS  Google Scholar 

  11. A. Miwa, J. Adachi, K. Mizuno, Y. Tatsuno, Clin. Chim. Acta258, 125 (1997).

    Article  CAS  Google Scholar 

  12. M. J. Paik, K. O. Lee, H. S. Shin, J. Chromatogr. B.721, 3 (1999).

    CAS  Google Scholar 

  13. Y. M. Lee, H. Nakamura, T. Nakajima, Anal. Sci.5, 681 (1989).

    CAS  Google Scholar 

  14. A. Takadate, T. Masuda, C. Tajima, C. Murata, M. Irikura, S. Goya, Anal. Sci.8, 663 (1992).

    CAS  Google Scholar 

  15. C. F. Chang, H. J. Wu, S. M. Wu, S. H. Chen, H. S. Kou, J. Liq. Chrom. & Rel. Technol.21 (5), 669 (1998).

    CAS  Google Scholar 

  16. T. Kobayash, M. Katayama, S. Suzika, H. Tomoda, I. Goto, Y. Kuroiwa, J. Neurol.230, 209 (1983).

    Article  Google Scholar 

  17. Y. Antoku, T. Sakai, I. Goto, H. Iwashita, Y. Kuroiwa, Neurology,34, 1499 (1984).

    CAS  Google Scholar 

  18. G. Kargas, T. Rudy, T. Spennetta, K. Takayama, N. Querishi, E. Shrago, J. Chromatogr.526, 331 (1990).

    CAS  Google Scholar 

  19. I. Zamir, E. Grushka, J. Chemke, J. Chromatogr.567, 319 (1991).

    CAS  Google Scholar 

  20. Y. Antoku, F. Koike, Y. Ohtsuka, T. Sakai, K. Tsukamoto, H. Nagara, H. Iwashita, I. Goto, Ann. Neurol.30, 101 (1991).

    Article  CAS  Google Scholar 

  21. T. Ohshima, K. I. Miyamoto, R. Sakai, J. Liq. Chromatogr.16 (15), 3217 (1993).

    CAS  Google Scholar 

  22. V. P. Dole, H. Meinert, J. Biol. Chem.235, 2559 (1960).

    Google Scholar 

  23. H. L. Wu, Y. Y. Shyu, H. S. Kou, S. H. Chen, S. M. Wu, S. S. Wu, J. Chromatogr A.769, 201 (1997).

    Article  Google Scholar 

  24. S. J. Lin, H. L. Wu, C. Y. Lin, Anal. Chim. Acta386, 113 (1999).

    Article  CAS  Google Scholar 

  25. J. Goto, in H. Lingeman, W.J.M. Underberg (Eds), Detection-oriented Derivatization Techniques in Liquid Chromatography, Marcel Dekker, New York, 1990, p. 338.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical College, 807, Kaohsiung, Taiwan

    Chi-Yu Lu, Hsin-Lung Wu, Su-Hwei Chen & Hwang-Shang Kou

Authors
  1. Chi-Yu Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hsin-Lung Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Su-Hwei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hwang-Shang Kou
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lu, CY., Wu, HL., Chen, SH. et al. A fluorimetric liquid chromatography for highly sensitive analysis of very long chain fatty acids as naphthoxyethyl derivatives. Chromatographia 51, 315–321 (2000). https://doi.org/10.1007/BF02490609

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02490609

Key Words

Search

Navigation