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Differential expression of tenascin in the skin during hapten-induced dermatitis

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Abstract

Tenascin is a large extracellular matrix glycoprotein which is found in limited regions of normal adult tissues including the skin. We investigated the induction of tenascin expression in mouse skin during hapten-in-duced dermatitis. In the dorsal skin, hapten application first induced a transient expression of tenascin in deeper regions of the skin. Its distribution then spread over the whole dermis corresponding to the infiltration of Mac-2-positive macrophages. In the ear, tenascin was consistently found in the subcutaneous tissue on the inner side, but very little was seen on the outer side. Tenascin did appear transiently, however, on both sides under hapten treatment. In the early phase of allergic contact dermatitis, no apparent induction of tenascin expression was observed in the swollen ear. However, there was an abundant tenascin expression on both sides during healing. Tenascin expressed under normal conditions was mostly the 180-kDa isoform, while the 230-kDa isoform was markedly induced during healing of the dermatitis. These results suggest that tenascin, particularly the larger 230-kDa isoform, may play important roles in the pathogenesis and healing of hapten-induced dermatitis.

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References

  • Anbazhagan R, Sakakura T, Gusterson BA (1990) The distribution of immuno-reactive tenascin in the epithelial-mesenchymal junctional areas of benign and malignant squamous epithelia. Virchows Arch 59:59–63

    Article  CAS  Google Scholar 

  • Aukhil I, Joshi P, Yan Y, Erickson HP (1993) Cell-and heparinbinding domains of the hexabrachion arm identified by tenascin expression proteins. J Biol Chem 268:2542–2553

    PubMed  CAS  Google Scholar 

  • Baar HMJ van, Perret CM, Vlijmen-Willems IMJJ van, Cleutjens JPM, Mackie EJ, Happle R, Schalkwijk J (1991) Expression of tenascin in perifollicular connective tissue: comparison of normal scalp and alopecia areata. Arch Dermatol Res 283:86–90

    Article  PubMed  Google Scholar 

  • Brown WR, Shivji GM (1990) Dose and timing studies for the optimization of contact sensitivity in the mouse. Acta Derm Venereol 71:44–47

    Google Scholar 

  • Chiquet-Ehrismann R, Kalla P, Pearson CA, Beck K, Chiquet M (1988) Tenascin interferes with fibronectin action. Cell 53:383–390

    Article  PubMed  CAS  Google Scholar 

  • Chuong CM, Chen HM (1991) Enhanced expression of neural cell adhesion molecules and tenascin (cytotactin) during wound healing. Am J Pathol 138:427–440

    PubMed  CAS  Google Scholar 

  • Erickson HP (1993) Tenascin-C, tenascin-R and tenascin-X: a family of talented proteins in search of functions. Curr Opin Cell Biol 5:869–876

    Article  PubMed  CAS  Google Scholar 

  • Hemesath TJ, Marton LS, Stefansson K (1994) Inhibition of T cell activation by the extracellular matrix protein tenascin. J Immunol 152:5199–5207

    PubMed  CAS  Google Scholar 

  • Hestdal K, Ruscetti FW, Ihle JN, Jacobsen SEW, Dubois CM, Kopp WC, Longo DL, Keller JR (1991) Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J Immunol 147:22–28

    PubMed  CAS  Google Scholar 

  • Hiraiwa N, Kida H, Sakakura T, Kusakabe M (1993) Induction of tenascin in cancer cells by interactions with embryonic mesenchyme mediated by a diffusible factor. J Cell Sci 104:289–296

    PubMed  CAS  Google Scholar 

  • Ho MK, Springer TA (1982) Mac-2, a novel 32,000 Mr mouse macrophage subpopulation-specific antigen defined by monoclonal antibodies. J Immunol 128:1221–1228

    PubMed  CAS  Google Scholar 

  • Lacour JP, Vitetta A, Chiquet-Ehrismann R, Pisani A, Ortonne JP (1992) Increased expression of tenascin in the dermis in scleroderma. Br J Dermatol 127:328–334

    Article  PubMed  CAS  Google Scholar 

  • Lightner VA, Gumkowski F, Bigner DD, Erickson HP (1989) Tenascin/hexabrachion in human skin: biochemical identification and localization by light and electron microscopy. J Cell Biol 108:2483–2493

    Article  PubMed  CAS  Google Scholar 

  • Mackie EJ, Halfter W, Liverani D (1988) Induction of tenascin in healing wounds. J Cell Biol 107:2757–2767

    Article  PubMed  CAS  Google Scholar 

  • Mackie EJ, Scott-Burden T, Hahn AWA, Kern F, Bernhardt J, Regenass S, Weller A, Buhler F (1992) Expression of tenascin by vascular smooth muscle cells: alterations in hypertensive rats and stimulation by angiotensin II, Am J Pathol 141:377–388

    PubMed  CAS  Google Scholar 

  • McCachren SS, Lightner VA (1992) Expression of human tenascin in synovitis and its regulation by interleukin-1. Arthritis Rheum 35:1185–1192

    PubMed  CAS  Google Scholar 

  • Medenica M, Rostenberg A Jr (1971) A comparative light and electron microscopic study of primary irritant contact dermatitis and allergic contact dermatitis. J Invest Dermatol 56:259–271

    Article  PubMed  CAS  Google Scholar 

  • Murphy-Ullrich JE, Lightner VA, Aukhil I, Yan YZ, Erickson HP, Hook M (1991) Focal adhesion integrity is downregulated by the alternatively spliced domain of human tenascin. J Cell Biol 115:1127–1136

    Article  PubMed  CAS  Google Scholar 

  • Oike Y, Hiraiwa H, Kawakatsu H, Nishikai M, Okinaka T, Suzuki T, Okada A, Yatami R, Sakakura T (1990) Isolation and characterization of human fibroblast tenascin. An extracellular matrix glycoprotein of interest for developmental studies. Int J Dev Biol 34:309–317

    PubMed  CAS  Google Scholar 

  • Pearson CA, Pearson D, Shibahara S, Hofsteenge J, Chiquet-Ehrismann R (1988) Tenascin: cDNA cloning and induction by TGF-β. EMBO J 7:2977–2982

    PubMed  CAS  Google Scholar 

  • Rettig WJ, Garin-Chesa P (1989) Cell type-specific control of human neuronectin secretion by polypeptide mediators and phorbol ester. J Histochem Cytochem 37:1777–1786

    PubMed  CAS  Google Scholar 

  • Rettig WJ, Triche TJ, Garin-Chesa P (1989) Stimulation of human neuronectin secretion by brain-derived growth factors. Brain Res 487:171–177

    Article  PubMed  CAS  Google Scholar 

  • Rettig WJ, Erickson HP, Albino AP, Grarin-Chesa P (1994) Induction of human tenascin (neuronectin) by growth factors and cytokines: cell type-specific signals and signalling pathways. J Cell Sci 107:487–497

    PubMed  CAS  Google Scholar 

  • Ruegg CR, Chiquet-Ehrismann R, Alkan SS (1989) Tenascin, an extracellular matrix protein, exerts immunomodulatory activities. Proc Natl Acad Sci USA 86:7437–7441

    Article  PubMed  CAS  Google Scholar 

  • Schalkwijk J, Vlijmen I van, Oosterling B, Perret C, Koopman R, Born J van den, Mackie EJ (1991a) Tenascin expression in hyperproliferative skin diseases. Br J Dermatol 124:13–20

    Article  PubMed  CAS  Google Scholar 

  • Schalkwijk J, Steijlen PM, Vlijmen-Willems JMJJ van, Oosterling B, Mackie EJ, Verstraeten AA (1991b) Tenascin expression in human dermis is related to epidermal proliferation. Am J Pathol 139:1143–1150

    PubMed  CAS  Google Scholar 

  • Sharifi BG, LaFleur DW, Pirola CF, Forrester JS, Fagin JA (1992) Angiotensin II regulates tenascin gene expression in vascular smooth muscle cells. J Biol Chem 267:23910–23915

    PubMed  CAS  Google Scholar 

  • Verstraeten AA, Mackie EJ, Hageman PHC, Hilgers J, Schol DJ, Jongh GJ de, Schalkwijk J (1992) Tenascin expression in basal cell carcinoma. Br J Dermatol 127:571–574

    Article  PubMed  CAS  Google Scholar 

  • Whitby DJ, Ferguson MWJ (1991) The extracellular matrix of lip wounds in fetal, neonatal and adult mice. Development 112:651–668

    PubMed  CAS  Google Scholar 

  • Yavin E, Gabai A, Gil S (1991) Nerve growth factor mediates monosialoganglioside-induced release of fibronectin and J1/tenascin from C6 glioma cells. J Neurochem 56:105–112

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Experimental Animal Research, Tsukuba Life Science Center, The Institute of Physical and Chemical Research, 3-1-1 Koyadai, 305, Tsukuba, Ibaraki, Japan

    Yoh-ichi Koyama, Masashi Kusubata & Moriaki Kusakabe

  2. Nippi Research Institute of Biomatrix, Nippi Inc., 1-1 Senju-Midoricho, Adachi-ku, 120, Tokyo, Japan

    Yoh-ichi Koyama, Keiko Norose, Masashi Kusubata & Shinkichi Irie

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  1. Yoh-ichi Koyama
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  2. Keiko Norose
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  3. Masashi Kusubata
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  4. Shinkichi Irie
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  5. Moriaki Kusakabe
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Koyama, Yi., Norose, K., Kusubata, M. et al. Differential expression of tenascin in the skin during hapten-induced dermatitis. Histochem Cell Biol 106, 263–273 (1996). https://doi.org/10.1007/BF02473236

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  • DOI: https://doi.org/10.1007/BF02473236

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