Abstract
The effects of degrading and stabilizing microtubules and microfilaments on the formation of plastic reactions were studied in isolated nerve cells from the molluskLymnaea stagnalis. Degradation of the cytoskeleton affected the performance, retention, and repeated acquisition of plastic reactions. Stabilization of microtubules led to the appearance of a relationship between the dynamics of the development and retention of plastic reactions and the series of stimulation. Stabilization of microfilaments led to transient plastic reaction, along with long-term reactions. These results show that rearrangements of the cytoskeleton have a key role in the processes of neuronal plasticity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
L. Sh. Ganelina and T. P. Nekrasova, “Protein kinase C and its role in normal and transformed cells,”Tsitologiya,31, No. 2, 131–147 (1989).
D. A. Moshkov,Neuron Adaptation and Ultrastructure [in Russian], Nauka, Moscow (1985).
A. S. Ratushnyak, R. A. Zapara, A. A. Zharkikh, and O. A. Tatushnyak, “The effects of changes in dynamic equilibrium in microtubule and microfilament systems on plastic reactions of neurons,”Zh. Vyssh. Nerv. Deyat.,46, No. 2, 355–362 (1995).
V. A. Tkachuk, “The role and position of cyclic nucleotides in the neuroendocrine regulation of cells and tissues,”Biol. Nauki,6, 5–17 (1987).
Yu. V. Chistyakova and E. V. Parfenova, “Ca protease—an enzyme involved in the metabolism of cytoskeletal proteins in the olfactory pavement of vertebrates,”Tsitologiya,11, 1345–1352 (1989).
Ch. Aoki and Ph. Siekevitz, “Ontogenetic changes in the cyclic adenosine-3,5-monophosphate-stimulatable phosphorylation of cat visual cortex proteins, particularly of microtubule-associated protein 2 (MAP2): effects of normal and dark rearing and of the exposure to light,”J. Neurosci.,5, No. 9, 2465–2483 (1985).
V. Bennet, K. Gardner, and J. Steiner, “Brain adducin: a protein kinase C substrate that may mediate site-directed assembly at the spectrin-actin junction,”J. Biol. Chem.,263, No. 12, 5860–5869 (1988).
M.-F. Carlier, D. Didry, and D. Pantalone, “Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover: Implication in actin-based motility,”J. Cell. Biol.,136, No. 6, 1307–1322 (1997).
N. A. Cohen, J. E. Brenman, S. H. Snyder, and D. S. Bredt, “Binding of the inward rectifier K+ channel Kir 2.3 to PSD-95 is regulated by protein kinase A phosphorylation,”Neuron,17, No. 4, 759–767 (1996).
M. R. C. Costa and W. A. Catterall, “Phosphorylation of the α-subunit of the sodium channel by protein kinase C,”Cell. Molec. Neurobiol.,4, No. 3, 291–297 (1984).
S. A. Deriemer, J. A. Strong, K. A. Albert, P. Greengard, and L. K. Haczmarek, “Enhancement of calcium current inAplysia neurones by phorbol ester and protein kinase C,Nature,313, 313–316 (1985).
D. A. Ewald, A. Williams, and I. B. Levitan, “Modulation of single Ca-dependent K-channel activity by protein phosphorylation,”Nature,315, No. 6019, 503–505 (1985).
J. Farley and S. Auerbach, “Protein kinase C activation induces conductance changes inHermissenda photoreceptors like those seen in associative learning,”Nature,319, No. 6050, 220–223 (1986).
S. Hameroff and R. Penrose, “Orchestrated reduction of quantum coherence in brain microtubules: A model for consciousness,”Mathemat. Comput. Simulat.,40, No. 3-4, 453–480 (1996).
B. D. Johnson and L. Byerly, “Ca channel Ca2+-dependent inactivation in a mammalian central neuron involves the cytoskeleton,”Pflügers Arch.,429, No. 1, 14–21 (1994).
M. Karin and T. Smeal, “Control of transcription factors by signal transduction pathway: the beginning of the end,”TIBS,17, 418–422 (1992).
A. Moon and D. G. Drubin, “The ADF/cofilin proteins: stimulus-responsive modulations of actin dynamics,”Mol. Biol. Cell.,6, 1423–1431 (1995).
E. J. Neer and D. E. Clapham, “Roles of G protein subunits in trans-membrane signalling,”Nature,333, No. 6169, 129–134 (1988).
L. S. Perlmutter, C. Gall, M. Baudry, and G. Lynch, “Distribution of calcium-activated and protease calpain in the brain,”J. Comp. Neurol.,296, No. 2, 269–276 (1990).
R. Prekeris, M. W. Mayhew, J. B. Cooper, and D. M. Terrian, “Identification and localization of an actin-binding motif that is unique to the epsilon isoform of protein kinase C and participates in regulation of synaptic function,”J. Cell. Biol.,132, No. 1-2, 77–90 (1996).
E. M. Quinlan and S. Halpaint “Emergence of activity-dependent, bidirectional control of microtubule-associated protein MAP2 phosphorylation during postnatal development,”Neurosci.,16, No. 23, 7627–7637 (1996).
C. Rosenmund and G. L. Westbrook, “Calcium-induced actin depolymerization reduces NMDA channel activity,”Neuron,10, No. 5, 805–814 (1993).
Ch. V. Schuster, G. W. Davis, R. D. Fitter, and C. S. Goodman, “Genetic dissection of structural and functional components of synaptic plasticity. II. Fasciclin II controls presynaptic structural plasticity,”Neuron 17, 655–667 (1996).
P. Vanderklish, T. C. Saido, C. Gall, A. Arai, and G Lynch, “Proteolysis of spectrin by calpain accompanies theta-burst stimulation in cultured hippocampal slices,”Brain Res. Mol. Brain,32, 25–35 (1995).
M. H. Wolf, H. Levine, W. S. May, P. Cuatrecasas, and N. A. Sahyoun, “A model for intracellular translocation of protein kinase C involving synergism between Ca and phorbol esters,”Nature,317, 546–549 (1985).
C. Wu, V. M. Keivens, T. E. O'Toole, J. A. McDonald, and M. H. Ginsberg, “Integrin activation and cytoskeletal interaction are critical steps in the assembly of a fibronectin matrix,”Cell,83, 715–724 (1995).
T. N. Yamoah and T. Crow, “Protein kinase and G-protein regulation of Ca currents inHermissenda photoreceptors by 5-HT and GABA,”J. Neurosci.,16, No. 15, 4799–4809 (1996).
Author information
Authors and Affiliations
Additional information
Translated from Rossiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 85, No. 1, pp. 128–138, January, 1999.
Rights and permissions
About this article
Cite this article
Zapara, T.A., Simonova, O.G., Zharkikh, A.A. et al. The effects of the dynamic state of the cytoskeleton on neuronal plasticity. Neurosci Behav Physiol 30, 347–355 (2000). https://doi.org/10.1007/BF02471789
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02471789