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In vitro growth and chemosensitivity studies of childhood cancers using clonogenic assay

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Abstract

We evaluated the usefulness of the recently developed Human Tumor Clonogenic Assay (HTCA) for chemosensitivity studies in childhood cancers. A total of 20 childhood cancer specimens containing 8 neuroblastomas, 4 malignant lymphomas, three hepatoblastomas, two rhabdomyosarcomas, two germinomas and one adrenal carcinoma were assayed. Overall, 65 per cent (13/20) of tumor cell specimens showed adequate colony forming ability to yield the sensitivity of anticancer drugs. The pattern ofin vitro sensitivity of a particular tumor to most anticancer drugs tested was not dissimilar to the clinical experiences of these agents. The overallin vitro response rate of childhood cancers to anticancer agents was 51 per cent. A retrospective analysis of this assay revealed a true positive rate of 57 per cent and a true negative rate of 100 per cent.

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Authors and Affiliations

  1. Department of Surgery, Nihon University School of Medicine, Itabashi-ku, 173, Tokyo, Japan

    Masahiko Shibata MD, Seigo Yasumuro, Wakato Ugajin, Yasuhiko Kurosu, Ikuo Okabe & Ken Morita

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  1. Masahiko Shibata MD
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  2. Seigo Yasumuro
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  3. Wakato Ugajin
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  4. Yasuhiko Kurosu
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  5. Ikuo Okabe
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  6. Ken Morita
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Shibata, M., Yasumuro, S., Ugajin, W. et al. In vitro growth and chemosensitivity studies of childhood cancers using clonogenic assay. The Japanese Journal of Surgery 16, 126–132 (1986). https://doi.org/10.1007/BF02471082

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  • DOI: https://doi.org/10.1007/BF02471082

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